Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 581 results for: BirA

Showing: 81 - 120 of 581 results

  1. pUAS-Myc-BirA-G3-ER

    Plasmid
    #173815
    Purpose
    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
    Depositor
    Norbert Perrimon
    Article
    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:
    Insert
    Myc-BirA-G3-ER
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    173815-plasmid-map-sequence-id-340316

    Enlarge

  5. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

    Enlarge

  11. pFBD-BirA_EIF2A:1-427

    Plasmid
    #210938
    Purpose
    Insect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    EIF2A:M1-T427 (EIF2A Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210938-plasmid-map-sequence-id-424569

    Enlarge

  12. pFBD-BirA_COPB2:1-300

    Plasmid
    #210917
    Purpose
    Insect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    COPB2:M1-G300 (COPB2 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210917-plasmid-map-sequence-id-424527

    Enlarge

  13. pFBD-BirA_PRPF4:1-522

    Plasmid
    #210896
    Purpose
    Insect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    PRPF4:M1-E522 (PRPF4 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210896-plasmid-map-sequence-id-424523

    Enlarge

  14. pFBD-BirA_WDR12:90-422

    Plasmid
    #210893
    Purpose
    Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR12:T90-G422 (WDR12 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210893-plasmid-map-sequence-id-424521

    Enlarge

  15. pFBD-BirA_WDR41:30-459

    Plasmid
    #210892
    Purpose
    Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR41:Q30-V459 (WDR41 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210892-plasmid-map-sequence-id-424520

    Enlarge

  16. pFBD-BirA_CORO1A:1-461

    Plasmid
    #210891
    Purpose
    Insect expression of full-length human CORO1A. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    CORO1A:M1-K461 (CORO1A Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210891-plasmid-map-sequence-id-424519

    Enlarge

  17. pFBD-BirA_WDR91:1-747

    Plasmid
    #210879
    Purpose
    Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR91:M1-A747 (WDR91 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210879-plasmid-map-sequence-id-424496

    Enlarge

  25. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Use
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

    Enlarge

  26. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid
    #36047
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag
    Depositor
    Kyle Roux
    Article
    Roux et al J Cell Biol. 2012 Mar 12.
    Has Service
    Cloning Grade DNA
    Insert
    BirA(R118G)-HA
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    R118G (highly promiscuous form)
    Promoter
    Available since
    April 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    36047-plasmid-map-sequence-id-381279

    Enlarge

  29. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

    Enlarge

  31. pMal-T-Avi-His/BirA

    Plasmid
    #102962
    Purpose
    Vector for in vivo biotinylation of a fusion protein in bacteria.
    Depositor
    Tonia Rex
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    102962-plasmid-map-sequence-id-199098

    Enlarge

  36. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Karl Kramer
    Type
    Empty backbone
    Use
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    53581-plasmid-map-sequence-id-242038

    Enlarge

  39. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid
    #118375
    Purpose
    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.
    Depositor
    Maria Vartiainen
    Article
    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs
    Type
    Empty backbone
    Use
    Destination
    Tags
    HA-BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118375-plasmid-map-sequence-id-229581

    Enlarge

Showing: 81 - 120 of 581 results