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Plasmid#222501PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSP2-48-merTetO-EFS-BLaR
Plasmid#118712PurposeContains an optimized 48-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-N22p-3XRFPnls
Plasmid#75387PurposeN22p-3XRFPnlsDepositorInsertN22p
UseLentiviralTags3XmCherryExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EFS-Cas9-P2A-HygR
Plasmid#164134PurposeLentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cellsDepositorInsertSpCas9 (cas9 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-3XGFPnls
Plasmid#75385PurposePCP-3XGFPnlsDepositorInsertPCP
UseLentiviralTags3XsfGFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)
Plasmid#229828PurposeA Piggybac compatible, cre dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-EFS-Zeo
Plasmid#227258PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0
Plasmid#153291PurposeExpress the adenosine sensor GRAB_Ado1.0 in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_ATP1.0
Plasmid#167581PurposeExpress the ATP sensor GRAB_ATP1.0 in Cre positive cellsDepositorInsertATP sensor GRAB_ATP1.0 (P2RY1 Human)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (S94A)
Plasmid#174169PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EFS-fDIO-GRAB_eCB2.0
Plasmid#164610PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterEFSAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM122: pAAV-EFS-CasRx
Plasmid#166870PurposeAAV vector for expressing CasRxDepositorInsertRfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFSAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EFS-MCS-WPRE
Plasmid#163362PurposeThe empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectorsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_NTS1.0
Plasmid#208671PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent mannerDepositorInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-dCas9-GFP
Plasmid#64104PurposeExpression of Sp dCas9-GFP in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EFS-Cas9-BlastR
Plasmid#115146PurposeSafe harbor site 231 knock-in vector with Cas9-BlastR expression cassetteDepositorInsertCas9-T2A-BlastR
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_Ado1.0med
Plasmid#153292PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cellsDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRFmut
Plasmid#208663PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_OTmut
Plasmid#185389PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent mannerDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF287_EFS-TiCas9-P2A-Puro
Plasmid#211646PurposeEFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.DepositorInsertTamoxifen-inducible SpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_CRF1.0
Plasmid#208662PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent mannerDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV-PGK-Cre-EFS-mScarletSIIN
Plasmid#172434PurposeLentivirus, expresses Cre recombinase and mScarlet-SIINFEKLDepositorInsertsCre
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP306-pAAV-EFS-MCS3-pA
Plasmid#113682PurposeEFS driven Multi Cloning Site-3.DepositorInsertN/A
UseAAVTagsExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-dHeFSpCas9-VP64-6xHis
Plasmid#92119PurposeExpression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive HeFSpCas9-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…PromoterT7Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJEP223-AAV-EFS-GFP-pA
Plasmid#62913PurposeAAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromotershort form of Elongation Factor1α (EFS)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.13.EFS-NS.H2B-RFP
Plasmid#170388PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2K7-RIP-Tomato-EFs-Tk::Sh
Plasmid#114311PurposeTissue-specific expression of the tdTomato living color under the control of the Rat Insulin 2 promoter (RIP)DepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HeFSpCas9-H840A
Plasmid#80456Purposehigh fidelity enhanced SpCas9 mammalian expression vectorDepositorInsertHeFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-hfCas13d-HA-mCherry-pA
Plasmid#233029PurposeTo Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoterDepositorInserthfCas13d-mCherry
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DjCas13d-HA-mCherry-pA
Plasmid#233030PurposeTo Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoterDepositorInsertDjCas13d-mCherry
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-CasRx-HA-mCherry-pA
Plasmid#233028PurposeTo Express HA tagged CasRX-mCherry fusion protein from the mammalian EFS promoterDepositorInsertCasRx-mCherry
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Zeo
Plasmid#227261PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1-24xms2
Plasmid#222968PurposeMS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequenceDepositorInsertFMR1-31CGG 5' UTR and exon1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p40651-EFSNS-FMR1_e1ΔCGG-24xms2
Plasmid#222969PurposeMS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequenceDepositorInsertFMR1-ΔCGG 5' UTR and exon1
UseTagsExpressionMammalianMutation31 CGG repeats deleted from 5' UTRPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EFS_mCherry2-NLS-PEST_Puro
Plasmid#213775PurposeFluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.DepositorInsertEFS_mCherry2-NLS-PEST
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF823_EFS-Cas9-P2A-Puro
Plasmid#211645PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF226_EFS-Cas9-P2A-Puro
Plasmid#211644PurposeEFS-Cas9-P2A-PuroDepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only