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Plasmid#166952PurposeLentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoterDepositorInsertKIF5A (Kif5a Mouse)
UseLentiviralTagsHAExpressionMutationPromotereF1aAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EVA1ext-hFcIgG3-IRES-YFP
Plasmid#64926PurposeTo express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)DepositorUseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterLTRAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEJS507-pCDest2-noAcr-mTagBFP2-IRES
Plasmid#85748PurposeExpresses mTagBFP2 only; used as a controlDepositorInsertmTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαWT-IRES-EGFP
Plasmid#183456PurposeReplacement of endogenous rat PKA-RIIα subunits with wild-type RIIαDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA2897_pPB-TREtight-UBCmin-cHA-IRESH2BmKO2
Plasmid#124176PurposepiggyBac-based Dox-inducible expression (moderate)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B IRES GFP
Plasmid#133817PurposeEncodes the C2B domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoterpax3a promoter -5.4kAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drd1a->M71-IRES-tauGFP ACNF TV
Plasmid#105073PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassetteDepositorUseMouse TargetingTagsExpressionMutationDrd1a coding sequence followed by IRES-tauGFP ACNFPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HD201: pMVP (L3-L2) IRES-eGFP + polyA
Plasmid#121747PurposepMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.DepositorInsertIRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
hPlekhm1-N/pIRES puro Glue (M1-A497)
Plasmid#73454PurposeExpress human N-terminal part (M1-A497) Plekhm1 in mammalian cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsCBP tag, Ha tag, and strep tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXCX-sGFAP-LMO-IRES-tdTomato
Plasmid#178312PurposeExpress the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytesDepositorInsertLactate 2-monooxygenase
UseAdenoviralTagstdTomatoExpressionMammalianMutationPromotersGFAPAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Tomato MEF2C S222A
Plasmid#89716PurposeRetroviral gene expression vector for MEF2C-S222A expressionDepositorInsertMEF2C (MEF2C Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-Polß(WT)--IRES-hyg
Plasmid#128667Purposemammalian expression of Flag-Polß(WT) protein with hygromycin selectionDepositorInsertPolB (POLB Human)
UseLentiviralTagsFlagExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-AICD-NES-IRES-hrGFP
Plasmid#107544PurposeAAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFPDepositorInsertAICD-NES (APP Human)
UseAAVTagsExpressionMutationNonePromoterCMVAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-S75F
Plasmid#24580DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationserine 75 changed to phenylalanine (codon TCT cha…PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-miRE_shRen-Blast
Plasmid#135684PurposeConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Blast
Plasmid#135686PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast
Plasmid#166412Purposeexpress hPOT1 K90E in mammalian cellsDepositorInserthPOT1 K90E (POT1 Human)
UseLentiviralTagsMYCExpressionMammalianMutationK90EPromoterEF-1aAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/UAA-mEGFP-IRES-mCherry
Plasmid#49234PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast POLD1 ISCmut
Plasmid#160806PurposeExpress ISC mutant POLD1DepositorInsertPOLD1 ISCmut (POLD1 Human)
UseRetroviralTagsExpressionMutationCodon optimized C-terminal 230 amino acids, C1058…PromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2A IRES GFP
Plasmid#133818PurposeEncodes the C2A domain of synaptotagmin 1 for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationPromoterhSyn (human synapsin I promoter)Available sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FLAG-RHEB-IRES-Puro
Plasmid#120576PurposeExpresses Flag-RHEB fusion protein in mammalian cells & for virus productionDepositorInsertRHEB (RHEB Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP
Plasmid#114731PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV floxed hMyoD-IRES-dsRed
Plasmid#66632Purposeco-expresses CRE excisable human MyoD and dsRedDepositorInserthuman MyoD IRES dsRed (MYOD1 Human)
UseCre/Lox and LentiviralTagsExpressionMutationPromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV T274V KSR1 IRES GFP
Plasmid#25977DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged threonine 274 to valinePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV S392A KSR1 IRES GFP
Plasmid#25978DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationchanged serine 392 to alaninePromoterAvailable sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Mc4r->M71-IRES-tauGFP ACNF TV
Plasmid#105072PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-333 of Mc4r and an IRES-tauGFP followed by ACNF cassetteDepositorInsertMc4r->M71-IRES-tauGFP-ACNF (Mc4r Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Beta2AR->M71-IRES-tauLacZ LNL TV
Plasmid#15621DepositorInsertBeta2AR->M71-IRES-tauLacZ LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV mouse Vav1DN IRES GFP
Plasmid#107092PurposeMammalian expression retroviral vectorDepositorInsertVav1 dominant negative cDNA (Vav1 Mouse)
UseRetroviralTagsExpressionMammalianMutationAA 1-186PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-A80T
Plasmid#24579DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationAlanine 80 changed to threonine (codon GCA to ACA)PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
M71->M72-IRES-tauGFP LNL TV
Plasmid#15641DepositorInsertM71->M72-IRES-tauGFP LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
MOR23Hybrid->M71-IRES-tauGFP LNL TV
Plasmid#15622DepositorInsertMOR23Hybrid->M71-IRES-tauGFP LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mouse-MycNV419D-IRES-RFP
Plasmid#73574PurposeMammalian expression of mouse MycN V419D mutantDepositorInsertMycn (Mycn Mouse)
UseRetroviralTagsIRES-RFPExpressionMammalianMutationV419DPromoterMSCVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-G139S
Plasmid#24581DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationGlycine 139 changed to serine (codon GGT to AGT)PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-S437R
Plasmid#24582DepositorInsertOfd1 (Ofd1 Mouse)
UseTagsMyc and eGFPExpressionMammalianMutationSerine 437 changed to Arginine (codon AGT to CGT)PromoterAvailable sinceJune 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP->M71-IRES-tauLacZ LNL TV
Plasmid#15627DepositorInsertGFP->M71-IRES-tauLacZ LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
M71::Myc6-IRES-taulLacZ LNL TV
Plasmid#15619DepositorInsertM71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
M71(N5Q)-IRES-tauLacZ LNL TV
Plasmid#15625DepositorInsertM71(N5Q)-IRES-tauLacZ LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
M71(deltaM1)-IRES-tauLacZ LNL TV
Plasmid#15624DepositorInsertM71(ΔM1)-IRES-tauLacZ LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
GFP->M72-IRES-tauLacZ LNL TV
Plasmid#15628DepositorInsertGFP->M72-IRES-tauLacZ LNL TV
UseHigh copy numberTagsExpressionMutationPromoterAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pT/CAGGS-NRASG12V/D38A-IRES-mVenus
Plasmid#236077PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).DepositorInsertsUseSleeping beauty transposon plasmidTagsExpressionMutationG12V D38APromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH
Plasmid#235294PurposeComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235270PurposeComMAND open-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235271PurposeComMAND closed-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2(miRE.FF4)-bGH
Plasmid#235289PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miRE.FF5)-bGH
Plasmid#235262PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miRE.FF4)-bGH
Plasmid#235288PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-miRE.FF4-bGH
Plasmid#235290PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only