We narrowed to 2,387 results for: plenti

1. plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

   Plasmid

   #69050

   Purpose

   lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   Q147H

   Promoter

   CAG

   Available since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

   Plasmid

   #236241

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV1.2 (CACNA1C Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

   Plasmid

   #236239

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.1 (CACNA1A Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

   Plasmid

   #236240

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.2 (CACNA1B Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-h.Rhno1 S141A (C)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211622

   Purpose

   Rhno1 S141A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S141A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211621

   Purpose

   Rhno1 T52A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   T52A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211620

   Purpose

   Rhno1 S51A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S51A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211624

   Purpose

   Rhno1 S163A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S163A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-h.Rhno1 S162A (D)-C-Myc-DDK-P2A-Puro

   Plasmid

   #211623

   Purpose

   Rhno1 S162A

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   hRHNO1 (RHNO1 Human)
   

   Use

   Tags

   myc-flag

   Expression

   Mammalian

   Mutation

   S162A

   Promoter

   CMV

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-h.Rhno1 S164A (F)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211625

    Purpose

    Rhno1 S164A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S164A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-h.Rhno1 S178A (G)-C-Myc-DDK-P2A-Puro

    Plasmid

    #211626

    Purpose

    Rhno1 S178A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc-flag

    Expression

    Mammalian

    Mutation

    S178A

    Promoter

    CMV

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

    Plasmid

    #211815

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and puromycin selection gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a promoter, U6 promoter, and hPGK promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204214

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DNA binding domain (delDBD)

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204191

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of R162

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204213

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on I207M

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204211

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on Q175* (*termination)

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204210

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Point mutation on R178* (*termination)

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204212

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NK2 homeobox 1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Point mutation on I207F

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti KO Luc Firefly 2xgRNA - spCas9 iRFP670 puro

    Plasmid

    #166134

    Purpose

    This plasmid allows efficient KO of the firefly luciferase gene, while expressing the far red fluorescent protein iRFP670 and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Deffieu et al Hepatology. 2022 Apr 7. doi: 10.1002/hep

    Insert

    hU6-gRNA and hH1-gRNA targeting firefly luciferase gene
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-3xFLAG-Puro PAK5 K478R/479R S364L

    Plasmid

    #167199

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R S364L; PAK7 Kinase dead & S364L double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R S364L
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-3xFLAG-Puro PAK5 K478R/479R D421N

    Plasmid

    #167200

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R D421N; PAK7 Kinase dead & D421N double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R D421N
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-3xFLAG-Puro PAK5 K478R/479R E294K

    Plasmid

    #167198

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E294K; PAK7 Kinase dead & E294K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E294K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-3xFLAG-Puro PAK5 K478R/479R M173I

    Plasmid

    #167197

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R M173I; PAK7 Kinase dead & M173I double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R M173I
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-3xFLAG-Puro PAK5 K478R/479R E144K

    Plasmid

    #167196

    Purpose

    puromycin resistant lentiviral vector for expression of PAK5 K478R/479R E144K; PAK7 Kinase dead & E144K double mutant

    Depositor

    Christin Burd

    Article

    LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

    Insert

    PAK5 K478R/479R E144K
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

    Plasmid

    #130678

    Purpose

    Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D409_F489del

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

    Plasmid

    #130691

    Purpose

    Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG and 3xHA

    Expression

    Mammalian

    Mutation

    E653_G1960del

    Promoter

    CMV

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

    Plasmid

    #130690

    Purpose

    Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

    Depositor

    Stephen Tapscott

    Article

    Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

    Insert

    SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    1M_T1522delinsM, G1720_Y1846del

    Promoter

    CMV

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167937

    Purpose

    Inducible CRISPRa expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    dxCas9(3.7)-VPR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid

    #138148

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

    Plasmid

    #177203

    Purpose

    encoding stblHIF under inducible promoter

    Depositor

    Markus Covert

    Article

    Kudo et al bioRxiv (2022)

    Insert

    stblHIF
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P402A/P577G/N813A

    Promoter

    TRE

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

    Plasmid

    #207832

    Purpose

    telomere specific nuclease to create double-strand breaks at telomeres

    Depositor

    Roger Greenberg

    Article

    Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag, DD, ER, mCherry and FokI

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

    Plasmid

    #233549

    Purpose

    A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas

    Plasmid

    #106171

    Purpose

    cre mediated red to green shift

    Depositor

    Ewa Snaar-Jagalska

    Article

    Heitzer et al Sci Rep. 2019 Mar 11;9(1):4096. doi: 10.

    Inserts

    dsRED-express2
    eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138145

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138146

    Purpose

    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PspCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

    Plasmid

    #199457

    Purpose

    Expression of dCas13-VPR in mammalian cells

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    dCas13
    

    Use

    Lentiviral

    Tags

    VPR-T2A-HaloTag

    Expression

    Mammalian

    Mutation

    Nuclease-dead H133A and H1058A

    Promoter

    CAG

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

    Plasmid

    #164229

    Purpose

    lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 235-314

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid

    #138143

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST

    Plasmid

    #164235

    Purpose

    lentiviral plasmid for GFP-TREX1 delta N (aa236-314)

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of amino acids 1-234

    Promoter

    CMV

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid

    #138144

    Purpose

    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

    Plasmid

    #202718

    Purpose

    Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Pink Flamindo
    paGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLenti-161K enhancer-ETS-CORE-MUT-minP:fLuc-TK:rLuc

    Plasmid

    #138367

    Purpose

    Lentiviral plasmid contains 161k modified human enhancer with with ETS binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    161K human enhancer, with ETS binding sites mutated, driving firefly enhancer and TK driving refills luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. JDW 1392 (pLenti-CMV-FOXF1-P2A-H2A-mCherry)

    Plasmid

    #233546

    Purpose

    A CMV driven human FOXF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF1 (FOXF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

    Plasmid

    #233547

    Purpose

    A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid

    #215012

    Purpose

    Express a ERphagy reporter in mammalian cells (untested)

    Depositor

    Wade Harper

    Insert

    KDEL
    

    Use

    Lentiviral

    Tags

    HaloTag(7)-mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro

    Plasmid

    #211616

    Purpose

    Rhno1 Plk1mut7A

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    hRHNO1 (RHNO1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S51A, T52A, S141A, S162A, S163A, S164A, S178A

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge