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pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

Plasmid

#61594

Purpose

A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pPS1494

Plasmid

#1377

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

pGAL-REV-GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

Plasmid

#61591

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS1372

Plasmid

#1353

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

pADH-NLS-NES-GFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pPS1523

Plasmid

#8919

Depositor

Pamela Silver

Article

Taura et al Proc Natl Acad Sci U S A 1998 Jun 23;95(

Insert

YRB2 (YRB2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

Plasmid

#61593

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

HA, NLS, and OLLAS tag

Expression

Mammalian

Mutation

K175R and K736R (deUb mutations)

Promoter

Available since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-hCRM1

Plasmid

#17647

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

CRM1 (XPO1 Human)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pTK21

Plasmid

#37396

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Ran (RAN Human)

Use

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

T24N

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-NPM NESM

Plasmid

#13282

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NLS1D

Plasmid

#13284

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NLS1/2D

Plasmid

#13287

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142, aa150-aa155

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NESD

Plasmid

#13283

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa94-aa102

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GFP-NPM NLS2D

Plasmid

#13285

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa150-aa155

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pAS404

Plasmid

#15978

Depositor

Eishi Noguchi

Article

Nakashima et al Genetics. 1999 Jul . 152(3):853-67.

Type

Empty backbone

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pDA313

Plasmid

#195214

Purpose

Expression of superfolder GFP (sfGFP) by a hybrid T7 promoter with tetO.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

sfGFP with tetO and a hairpin

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Rap2b

Plasmid

#118321

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-RapGAP

Plasmid

#118324

Purpose

Expression of RapGAP

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

RapGAP

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge