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pLCHKO_MDM4_exon_deletion_3

Plasmid

#155075

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Jason Moffat, Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Sxl].1046B

Plasmid

#112692

Purpose

express two gRNA targeting bTub & Sxl under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PRKRA gRNA (BRDN0001146443)

Plasmid

#76299

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKRA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKRA (PRKRA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-sgSmad4#1/Cre

Plasmid

#173617

Purpose

Expresses a Smad4-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Smad4 (Smad4 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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ZAP70 gRNA (BRDN0001487050)

Plasmid

#77956

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZAP70

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ZAP70 (ZAP70 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMCh2007_pLenti_Syn_mChe-cdc42E7

Plasmid

#118620

Purpose

expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed cagtctg to ggcataa (667 - 673 nt in 3…

Promoter

synapsin I (rat)

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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TRRAP gRNA (BRDN0001144900)

Plasmid

#76537

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRRAP

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRRAP (TRRAP Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUDR217

Plasmid

#113872

Purpose

expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

Depositor

Jack Pronk, Jean-Marc Daran

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA9-MPH2-3 sgRNA10-MAL11

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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MPP1 gRNA (BRDN0001148092)

Plasmid

#77805

Purpose

3rd generation lentiviral gRNA plasmid targeting human MPP1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MPP1 (MPP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PBK gRNA (BRDN0001145450)

Plasmid

#75572

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PBK (PBK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128337

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna2 (Chrna2 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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TRIM24 gRNA (BRDN0001148301)

Plasmid

#77888

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM24

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PPIP5K2 gRNA (BRDN0001148255)

Plasmid

#76341

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPIP5K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PPIP5K2 (PPIP5K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIP4K2B gRNA (BRDN0001147019)

Plasmid

#77903

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP4K2B

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP4K2B (PIP4K2B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PPIP5K1 gRNA (BRDN0001148626)

Plasmid

#76308

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPIP5K1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PPIP5K1 (PPIP5K1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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RAB11FIP5 gRNA (BRDN0001148210)

Plasmid

#76185

Purpose

3rd generation lentiviral gRNA plasmid targeting human RAB11FIP5

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

RAB11FIP5 (RAB11FIP5 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MVK gRNA (BRDN0001162405)

Plasmid

#77406

Purpose

3rd generation lentiviral gRNA plasmid targeting human MVK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MVK (MVK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCZ gRNA (BRDN0001145473)

Plasmid

#76631

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCZ

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCZ (PRKCZ Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKMT1A gRNA (BRDN0001145999)

Plasmid

#75878

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKMT1A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKMT1A,CKMT1B (CKMT1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pk335-CARGO-11mer-35kb-DSF-1-11

Plasmid

#227489

Purpose

11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

CARGO to 35kb Downstream Fgf5

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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