We narrowed to 59,655 results for: plasmid
-
Plasmid#43918DepositorUseLentiviral; Doxycycline inducibleTags6xHis and V5ExpressionMammalianMutationGylcine-serine-glycine (GSG) linker followed by v…PromoterTRE promoter, Tet-ONAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by
-
pRRL_SRSF2_WT_mCherry
Plasmid#84020Purposeexpression of SRSF2 in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry
Plasmid#44362PurposeDouble floxed Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV RetrogradeInserthM4D(Gi)-mCherry
UseAAV; Adeno associated viral vectorTagsmCherryPromoterhuman Synapsin 1Available sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCmini-iCAP-AAV9-X1.1
Plasmid#196836Purposenon-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV9-X1.1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pnCasPA-BEC
Plasmid#113349PurposeA cytidine deaminase-mediated base-editing plasmid in Pseudomonas speciesDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PT3.5/CMV-EGFRvIII
Plasmid#20280DepositorAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inse…Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SBE4-Luc
Plasmid#16495PurposeLuciferase reporter containing four copies of the Smad binding siteDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP670nano3
ExpressionMammalianPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRfx-Cas13d
UseCRISPRTagsHAAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cre Stoplight 2.4
Plasmid#37402PurposeCre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with CreDepositorInsertCre Stoplight 2.4
TagsZsGreen1 and mCherryExpressionMammalianPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSaCas9_GFP
Plasmid#64709PurposeCo-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSaCas9-2A-GFP
UseCRISPRTags2A-GFP and 3xFLAG-SV40 NLSExpressionMammalianPromoterCAGAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET23b-15F11-HA-mEGFP-6xHis
Plasmid#129593PurposeEncodes anti-HA scFv (anti-HA frankenbody) fused with mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
Tags6xHis-tagExpressionBacterialPromoterT7Available sinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
TagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-puro LEF1
Plasmid#27023DepositorPublicationAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLp6.3-Reverse
Plasmid#79117PurposeLentiviral expression of EglN3-ReverseDepositorInsertcontrol plasmid with reverse EglN3 insert (EGLN3 Human)
UseLentiviralTagsV5ExpressionMammalianPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF1389
Plasmid#104962PurposeBacterial expression plasmid of bdSENP1 proteaseDepositorInsertSENP1
TagsHis14 tag and Tev protease cleavage siteExpressionBacterialPromoterlacUV5Available sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterMaize ubiquitin 1 (ZmUbi1)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by