163,682 results

1. Geminin-mStrawberry reporter

   Plasmid

   #158690

   Purpose

   This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-sh-hSOX9-1

   Plasmid

   #40644

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-hSOX9-1 (SOX9 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. KA1153_pPBCAG-MerCreMer-IN

   Plasmid

   #124183

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Neo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS648∙CsR

   Plasmid

   #197860

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Gm resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available Since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB554

   Plasmid

   #74884

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIPZ-Wt-PTEN

   Plasmid

   #206982

   Purpose

   Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

   Depositor

   Ming Chen

   Article

   Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on promoter

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-C-TEV-Twin-Strep

   Plasmid

   #202527

   Purpose

   Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1-GFP

    Plasmid

    #180420

    Purpose

    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.

    Depositor

    Nancy Moran

    Article

    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PresentER-Cassette GFP

    Plasmid

    #102942

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

    Plasmid

    #71293

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Use

    Tags

    FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP3664

    Plasmid

    #222350

    Purpose

    Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LbABE8e(TadA-8e V106W)

    Plasmid

    #138508

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherry-C1-MKLP1

    Plasmid

    #70154

    Purpose

    mammalian expression of MKLP1 with an mCherry fusion

    Depositor

    Masanori Mishima

    Article

    Joseph et al EMBO J. 2012 May 30;31(11):2604-14. doi:

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. mPlum-C1

    Plasmid

    #54839

    Purpose

    Localization: C1 Cloning Vector, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZT-AAVS1-R1

    Plasmid

    #52638

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Right AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Syn-PSD95-mCherry

    Plasmid

    #125694

    Purpose

    Exogenous expression of PSD95 (red fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pnEA-vH_His-TEV-SEPT2-msfGFP

    Plasmid

    #174492

    Purpose

    bacterial expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-EGFP-Rac1-T17N

    Plasmid

    #12982

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 DN (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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24. pFastBacDual with PA+Histag

    Plasmid

    #84870

    Purpose

    Baculovirus Expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Baculovirus

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pFUW H2B-mTagBFP2-P2A-PuroR

    Plasmid

    #239552

    Purpose

    Expression and nuclear localization of mTagBFP2

    Depositor

    Tim Ahfeldt, Joel Blanchard

    Article

    et al iScience 28 (2025)

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UbC

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEJS2422_Nme2.Smu-ABE8e-i1

    Plasmid

    #201520

    Purpose

    Mammalian expression of domain inlaid Nme2.Smu-ABE8e-i1

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2.Smu-ABE8e-i1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZT-AAVS1-L1

    Plasmid

    #52637

    Purpose

    TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

    Depositor

    Mahendra Rao, Jizhong Zou

    Article

    Luo et al Stem Cells Transl Med. 2014 May 15. pii:

    Insert

    Left AAVS1 TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCD8-GFP in pUAST

    Plasmid

    #17746

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    mouse CD8 (Cd8a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge