159,557 results

1. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSK9065

   Plasmid

   #84345

   Purpose

   tightly controlled expression of mRFP fusion proteins in S. aureus

   Depositor

   Neville Firth

   Article

   Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

   Type

   Empty backbone

   Use

   Tags

   mRFPmars

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl/tet

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. VCP(DK0)-EGFP

   Plasmid

   #23974

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Use

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   E305QE578Q

   Promoter

   Available since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX208-beta-arrestin2-HA-TEV219

   Plasmid

   #104846

   Purpose

   b2AR-SPARK protease component

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   beta-arrestin2-HA-TEV219
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp6-mNeongreen

   Plasmid

   #182388

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-mNeongreen-His
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN1-CMV-mCherryN-IntN-DrBphP (N23)

   Plasmid

   #210724

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   N23
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-EGFP-NLS

   Plasmid

   #165441

   Purpose

   Expresses nuclear EGFP from CMV promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

   Insert

   EGFP-NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK-Hygro

   Plasmid

   #25737

   Purpose

   3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1-TetOn-dCas9-KRAB

    Plasmid

    #115545

    Purpose

    Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

    Depositor

    Rene Maehr

    Article

    Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Kaede

    Plasmid

    #132972

    Purpose

    full-length of photoconvertible kaede with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    Kaede
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. APOE_Halo_C_allele

    Plasmid

    #178121

    Purpose

    Donor vector for endogenous tagging of human APOE at the C-terminus with halotag

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Halotag (APOE Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_051_dCas9-KRAB-HA

    Plasmid

    #228936

    Purpose

    All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSP3545

    Plasmid

    #46888

    Purpose

    Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

    Depositor

    Gary Dunny

    Article

    Bryan et al Plasmid. 2000 Sep;44(2):183-90.

    Type

    Empty backbone

    Use

    Nisin-inducible expression; gram-positive bacteri…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PnisA

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE3G-Bi-α-chain

    Plasmid

    #133730

    Purpose

    Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    a-Chain_gePSI
    ZsGreen1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Only one part of the gePSI-system (aa1-141 + stop…

    Promoter

    pTRE3G-Bi

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPOTv7-blast-10V5

    Plasmid

    #181938

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    10xV5
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    read through

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-UtrCH

    Plasmid

    #26737

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261).…

    Promoter

    CMV IE94

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNLS-iRFP713

    Plasmid

    #45468

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAV0279

    Plasmid

    #149688

    Purpose

    Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SUMO_Eu1-MBP
    

    Use

    Tags

    3xFLAG-tag and His14-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pPROEX-TwStrp-FTO

    Plasmid

    #190265

    Purpose

    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.

    Depositor

    Samie Jaffrey

    Insert

    FTO (FTO Human)
    

    Use

    Tags

    6xHis and Twin-Streptag

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EGFP-CLC

    Plasmid

    #193559

    Purpose

    Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

    Depositor

    Justin Taraska

    Article

    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

    Insert

    Clathrin light chain B (CLTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-HsSENP3

    Plasmid

    #71467

    Purpose

    Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-pCAG-donor(R2Tg)-GFP(intron)

    Plasmid

    #223248

    Purpose

    Mammalian expression of the R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET22b-CPDBamHI-Leu

    Plasmid

    #38256

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-PTK2-C-10

    Plasmid

    #55122

    Purpose

    Localization: Focal Adhesion Kinase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pTKRED

    Plasmid

    #41062

    Depositor

    Edward Cox, Thomas Kuhlman

    Article

    Kuhlman et al Nucleic Acids Res. 2010 Apr;38(6):e92. E

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge