We narrowed to 14,011 results for: flag

1. pAAV-CBh-DIO-CB2-EGFP

   Plasmid

   #91946

   Purpose

   Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Inserts

   EGFP
   type-2 cannabinoid receptor
   

   Use

   AAV

   Tags

   FLAG and None

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLP-LNCX-PIK3CA-WT

   Plasmid

   #25633

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

3. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

   Plasmid

   #135460

   Purpose

   Mammalian expression of uFLARE TF component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
   

   Use

   Tags

   HA, flag,

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-Pidd1(T788A)

   Plasmid

   #60529

   Purpose

   Retroviral expression of Pidd1 T788A phosphorylation mutant

   Depositor

   Trudy Oliver

   Article

   Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

   Insert

   Pidd1 (PIDD1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Threonine 788 to Alanine*

   Promoter

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pJW1354

   Plasmid

   #69490

   Purpose

   Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

   Depositor

   Jordan Ward

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
   

   Use

   CRISPR; Template to generate cassettes for -media…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. C204

   Plasmid

   #174358

   Purpose

   For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   PABPC1 (PABPC1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Multiple silent mutations introduced to be resist…

   Promoter

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LPCX Smad2

   Plasmid

   #12636

   Depositor

   Rik Derynck

   Article

   Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-NPM NESM

    Plasmid

    #13282

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L100A, L102A

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-NPM NLS1D

    Plasmid

    #13284

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa134-aa142

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCAG APX W41F dimeric (mito matrix)

    Plasmid

    #40307

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    Flag

    Expression

    Mutation

    W41F mutation.

    Promoter

    CAG

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. PAX6 TALEN R

    Plasmid

    #105525

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    Pax6 TALEN R
    

    Use

    TALEN

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAX6 TALEN L

    Plasmid

    #109034

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    Pax6 TALEN L
    

    Use

    TALEN

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWB-TRAF2

    Plasmid

    #44114

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available Since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

17. GFP-NPM NLS1/2D

    Plasmid

    #13287

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa134-aa142, aa150-aa155

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

18. Erk1

    Plasmid

    #29578

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Tags

    FLAG, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    K289N mutation present. Starting Met removed from…

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-NPM NESM-NLS1D

    Plasmid

    #13288

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L100A, L102A, deletion of aa134-aa142

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

21. pRG710_lexO-HO_LexA-TF_HIS3MX

    Plasmid

    #154819

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta1

    Plasmid

    #106114

    Purpose

    Lentiviral expression of PRKRA delta1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and myc

    Expression

    Mutation

    AA 100-313

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDNA-FRT-FAK-kinase30

    Plasmid

    #59124

    Purpose

    FAK fret control sensor without FERM domain with 30 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ac5-STABLE2-blast

    Plasmid

    #208034

    Purpose

    Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta23

    Plasmid

    #106117

    Purpose

    Lentiviral expression of PRKRA delta23

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    PRKRA (PRKRA Human)
    

    Use

    Lentiviral

    Tags

    DDK (FLAG) and myc

    Expression

    Mutation

    AA 1-127

    Promoter

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-NPM NESD

    Plasmid

    #13283

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    NPM (NPM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of aa94-aa102

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

29. pMSCV MEKK3 PB1

    Plasmid

    #12185

    Depositor

    Gary Johnson

    Article

    Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

    Insert

    MEKK3 (Map3k3 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PB1 domain: aa 36-127

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pSplit-OC-U5-40K

    Plasmid

    #51741

    Purpose

    Express U5-40K-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    U5-40K (SNRNP40 Human)
    

    Use

    Tags

    FLAG and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only