We narrowed to 10,120 results for: rras

1. pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

   Plasmid

   #223215

   Purpose

   Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   mCherry BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. PDIA5-bio-His

   Plasmid

   #52072

   Purpose

   Expresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PDIA5 (PDIA5 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-DAXX-mSIM1/2

   Plasmid

   #134525

   Purpose

   Expresses YFP-DAXX fusion protein with a mutations in both its 5' and 3' SIMs

   Depositor

   Susan Janicki

   Article

   Shastrula et al J Cell Sci. 2019 Mar 26;132(6). pii: jcs

   Insert

   DAXX-mSIM1/2
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   SIM (IIVL) 7-10 aa changed to IGAG and SIM (IIVL)…

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-GRAB_eCBmut

   Plasmid

   #164605

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S383T, F177A

   Promoter

   hSyn

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC10-EFS-huLbCpf1-2A-Blast-WPRE

   Plasmid

   #123359

   Purpose

   Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

   Plasmid

   #175020

   Purpose

   Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

   Insert

   D/bovine/Nebraska/9-5/2012
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   s57a esterase knock out mutant

   Promoter

   CMV

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ULBP3-bio-His

   Plasmid

   #51859

   Purpose

   Expresses full-length NKG2D ligand 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ULBP3 (ULBP3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

   Plasmid

   #123362

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Firefly luciferase
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. U6-CjCas9-MS2gRNA-Backbone

    Plasmid

    #210711

    Purpose

    This plasmid express MS2 loop containing Cj Cas9 gRNA

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MS2 loop containing Cj Cas9 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB12A

    Plasmid

    #210035

    Purpose

    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas12a
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-FLAG-MYO1D

    Plasmid

    #125125

    Purpose

    Expresses human FLAG-MYO1D

    Depositor

    Bruce Beutler

    Article

    McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

    Insert

    MYO1D (MYO1D Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-fDIO-GRAB_eCB2.0

    Plasmid

    #164610

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    EFS

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-DIO-GRAB_eCB2.0mut

    Plasmid

    #164607

    Purpose

    Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid non-responsive sensor GRAB_eCBmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T, F177A

    Promoter

    hSyn

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN-2xFKBP-mEGFP-NDP52

    Plasmid

    #110465

    Purpose

    retrovirus construct for stable expressing mEGFP tagged NDP52

    Depositor

    Richard Youle

    Article

    Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral; Retrovirus

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEND-193_Cacnes_HS

    Plasmid

    #225622

    Purpose

    C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    HspR
    

    Use

    Synthetic Biology

    Tags

    pFAST-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG_mIGF1R_EGFP

    Plasmid

    #199629

    Purpose

    mutant FRET donor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    mIGF1R (IGF1R Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Y1316F

    Promoter

    CAG

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-empty-DSE-TetO-8kb

    Plasmid

    #213022

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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