We narrowed to 11,564 results for: phen

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pHEA

Plasmid

#168297

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

Insert

Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)

Tags

6xHis, E-tag, and PelB signal peptide

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pKM197

Plasmid

#132665

Purpose

pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

Depositor

Joseph Sorg

Article

Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

Insert

Upstream & Downstream pyrE deletion region

Use

E. coli - c. difficile shuttle vector

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMemoryArray

Plasmid

#60585

Purpose

An array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

MemoryArray

Expression

Bacterial

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-Tandem-mCherry-EGFP

Plasmid

#123216

Purpose

N-terminal Tandem mCherry EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_023

Plasmid

#123715

Purpose

Encodes pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pnCas9-AID

Plasmid

#196242

Purpose

Plasmid expressing the nCas9-AID fusion

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

nickase Cas9 fused to AID

Use

CRISPR

Expression

Bacterial

Promoter

tac promoter

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLCS105

Plasmid

#209358

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-tglB

Plasmid

#134676

Purpose

Expresses His6-TglBin E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglB

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDest-635

Plasmid

#159582

Purpose

Gateway Destination vector for protein expression in baculovirus

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLG016 (ApeA)

Plasmid

#157894

Purpose

Expresses the ApeA defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ApeA (putative HEPN RNase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIH CMV/TO DEST (w382-1)

Plasmid

#17394

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL6090

Plasmid

#193142

Purpose

Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Cbe ZTP Riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Mutation

Deleted A26 to disable ligand-binding

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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