19,658 results

1. Flag-neo-PP1-Alpha

   Plasmid

   #171267

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3x-Flag-tagged human PP1 alpha catalytic subunit (PPP1CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-myc-Cdc42-T17N

   Plasmid

   #12973

   Depositor

   Gary Bokoch

   Insert

   Cdc42 DN (CDC42 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac2.tR4-OMeYRS/GFP*

   Plasmid

   #50831

   Purpose

   baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells

   Depositor

   Peter Schultz

   Article

   Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Inserts

   two-copy Tyr tRNA cassette
   EGFP*
   OMeYRS
   two copy Tyr tRNA cassette
   

   Use

   Baculoviral

   Tags

   His, Myc-6xHis, and WPRE

   Expression

   Insect

   Mutation

   Y39TAG and able to charge various unnatural amino…

   Promoter

   CAG, CMV, and U6 and H1

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. LBR pEGFP-N2 (646)

   Plasmid

   #61996

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   LBR (LBR Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. MLRV

   Plasmid

   #118069

   Purpose

   Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Inserts

   TB:5xNF-κβ:RedF:bGHpA
   TB:4xTGF-β:FLuc:bGHpA
   TB:5xE-box:Renilla:bGHpA
   TB:2xp53:NLuc:bGHpA
   TB:6xAP-1:GrRenilla:bGHpA
   CMV:ELuc:bGHpA
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Promoter

   2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AB-GFP

   Plasmid

   #72203

   Purpose

   screen AB42 variants for aggregation

   Depositor

   Michael Hecht

   Article

   Wurth et al J Mol Biol. 2002 Jun 21;319(5):1279-90.

   Insert

   AB42 (APP Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Rab5a-7

   Plasmid

   #55126

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab5a
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestattB/CY (pCM327)

    Plasmid

    #68341

    Purpose

    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Type

    Empty backbone

    Use

    Zebrafish transgenesis

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Expression

    Bacterial

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-hM3Dq-mCherry

    Plasmid

    #166599

    Purpose

    Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCB-hDot1Lmut

    Plasmid

    #74174

    Purpose

    For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

    Depositor

    Yi Zhang

    Article

    Okada et al Cell. 2005 Apr 22;121(2):167-78.

    Insert

    hDot1L (DOT1L Human)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    hDot1L 163 GSG to RCR mutation*

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

16. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAXEF-Rap1-WT

    Plasmid

    #32697

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Expression

    Mammalian

    Promoter

    EF promoter

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. AbVec2.0-mIgkc

    Plasmid

    #127157

    Purpose

    Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Igkc (Igkc Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. HDAC4 Flag

    Plasmid

    #13821

    Purpose

    Mammalian expression of human histone deacetylase 4 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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