12,939 results

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
SEMA3C (mouse) pBLUE

Plasmid

#29451

Depositor

Jonathan Raper

Insert

SEMA3C (Sema3c Mouse)

Use

Pblue for ish

Mutation

for in situ hybridization

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
mEos3.2trLAT-2P

Plasmid

#203748

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLAToC27AC30A (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Mutation

C27A, C30A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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FTCAR2:pFlagCMV-mCAR-TVV

Plasmid

#87239

Purpose

Mammalian expression of mouse CAR, TVV isoform

Depositor

Fritz Rathjen

Insert

CXADR (Cxadr Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMyt1GFPicre-Knock-In

Plasmid

#22657

Depositor

Lynn Hudson

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Targeting vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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TRPML3-MYC

Plasmid

#18833

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6-H133Y

Plasmid

#20279

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Histidine 133 to Tyrosine

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pEGFP-Map7emtb-Kif5bmotor

Plasmid

#46077

Depositor

Edgar Gomes

Article

Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

Insert

Kif5bmotor-Map7emtb

Tags

GFP

Expression

Mammalian

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17S

Plasmid

#35414

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17s (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-tagBFP

Plasmid

#65254

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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Px458-Cas9n-Trp63-Exon4-1sgRNA

Plasmid

#88848

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63 (Trp63 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pUC19 PKC zeta

Plasmid

#8425

Depositor

Frederic Mushinski

Article

Goodnight et al Gene 1992 Dec 15;122(2):305-11.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
Sema4b-Fc-His

Plasmid

#72155

Purpose

Expresses the extracellular region of the Sema4B protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4b (Sema4b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-Fah_W

Plasmid

#163087

Purpose

expressing mouse wild type FAH in mammalian cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Insert

Fah_W

Tags

Myc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1-AA

Plasmid

#160942

Purpose

Generation of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Change Cys 287 Glu 288 into Ala Ala

Promoter

MSCV-LTR

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p248_LTJ_2kbCD45.1Template

Plasmid

#82662

Purpose

2kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Ankyrin-B [N105/13R]

Plasmid

#177493

Purpose

Mammalian Expression Plasmid of anti-Ankyrin-B (Human). Derived from hybridoma N105/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Ankyrin-B (Homo sapiens) recombinant mouse monoclonal antibody (ANK2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK2 KD

Plasmid

#12183

Depositor

Gary Johnson

Article

Fanger et al EMBO J. 1997 Aug 15. 16(16):4961-72.

Insert

MEKK2 (Map3k2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K385M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
LDN

Plasmid

#21195

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Human, Mouse)

Use

Retroviral

Mutation

Mek1 K97M fused to ER*, catalytically inactive

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only