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pAAV-hSyn-GRAB_DA1m

Plasmid

#113049

Purpose

express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1m in neurons

Depositor

Yulong Li

Publication

Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

Has service

AAV9

Insert

GPCR activation based DA sensor GRAB_DA1m (DRD2 Human)

Use

AAV

Promoter

hSyn

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Publication

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Publication

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

Plasmid

#103849

Purpose

dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Promoter

CMV

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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htrpC6-YFP

Plasmid

#21084

Depositor

Craig Montell

Publication

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TrpC6 (TRPC6 Human)

Tags

YFP

Expression

Mammalian

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTubb3-MC

Plasmid

#87112

Purpose

minicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITI

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Use

CRISPR

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Publication

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

Plasmid

#67986

Purpose

Cas9 activity reporter with mCherry and BFP

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Inserts

U6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Publication

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Tandem Dimer smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3

Plasmid

#78804

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLentiCRISPR-tagBFP

Plasmid

#75160

Purpose

Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

Depositor

Beat Bornhauser

Publication

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

tagBFP

Expression

Mammalian

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Publication

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Publication

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTNS1

Plasmid

#64967

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Available since

Oct. 23, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Publication

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Tags

His

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RGS-6xHis-pcDNA3.1-

Plasmid

#52534

Purpose

enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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