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pLENTI_RPS10_K139R

Plasmid

#127137

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K139R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10_K138R

Plasmid

#127136

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K138R

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pReporter_4

Plasmid

#60565

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_4

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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14UAS zfPSD95:GFP

Plasmid

#74314

Purpose

A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Insert

zebrafish PSD95

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Promoter

UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pAC-EsaR

Plasmid

#47643

Purpose

wt esaR gene under control of Plac in pACYC184

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

Plac-esaR-terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR; Gateway destination

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Article

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pltetO1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRO174

Plasmid

#233809

Purpose

MoClo-YTK; type3A; ABA systems; Phlf-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Phlf-NES

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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LbCas12a-gRNA_BbsI_CAM

Plasmid

#183222

Purpose

LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

LbCas12a guide RNA containing BbsI sites in spacer sequence

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
MTK2_005

Plasmid

#123700

Purpose

Encodes pCAG as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCAG

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pBL4

Plasmid

#50548

Purpose

Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Inserts

CcaS
CcaR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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BPK2014-Cas12i-Max

Plasmid

#188276

Purpose

prokaryotic expression

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i-Max

Use

CRISPR

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pReporter_12

Plasmid

#60572

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_12

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE G1

Plasmid

#221978

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE C2

Plasmid

#221979

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE T1

Plasmid

#221980

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ND4 DdCBE T2

Plasmid

#221981

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge