19,815 results

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pDsRed-Sensor

Plasmid

#22743

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

modified pCMV-DsRed-Express (Clontech)

Expression

Mammalian

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

hSynapsin

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCas12f1

Plasmid

#171182

Purpose

Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

Cas12f1

Use

CRISPR

Expression

Bacterial

Promoter

cas9 promoter from Streptococcus pyogenes

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-WDR5

Plasmid

#29759

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

WDR5 (WDR5 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5 BRGI-Flag

Plasmid

#19143

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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AAV2-miniSOG-VAMP2-T2A-mCherry

Plasmid

#50970

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-T2A-mCherry (Vamp2 Mouse, Arabdopsis thaliana)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Hexim2 (P#1897)

Plasmid

#14663

Depositor

Matija Peterlin

Article

Barboric et al EMBO J. 2005 Dec 21. 24(24):4291-303.

Insert

Hexim2 (HEXIM2 Human)

Tags

His and Xpress

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP7f

Plasmid

#104483

Purpose

Mammalian expression of ultrasensitive protein calcium sensor

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7f

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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Pseudojanin (PJ)

Plasmid

#37999

Depositor

Robin Irvine

Article

Hammond et al Science. 2012 Jun 21.

Inserts

FKBP1A (FKBP1A Human)
SAC1
INPP5E (INPP5E Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pBBR1k-con-GFPuv

Plasmid

#106386

Purpose

Constitutive trc promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

trc

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP

Plasmid

#27704

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlpha15-RLuc8

Plasmid

#140984

Purpose

Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlpha15-RLuc8 (GNA15 Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAG424GAL-ccdB

Plasmid

#14151

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Expression

Mammalian

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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