59,541 results

1. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/TO/HIS/FLAG/HA-MYBBP1A

   Plasmid

   #38084

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   MYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
   

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET32a-HD46Q

   Plasmid

   #11515

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 46Q glutamines (HTT Human)
   

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 46 glutamines, vector m…

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. CL20_mEGFP_EWSR1_ERG

   Plasmid

   #205819

   Purpose

   Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EWSR1_ERG (EWSR1, ERG Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FL-BCAN-lentiviral-GFP

   Plasmid

   #29704

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   Brevican (BCAN Human)
   

   Use

   Lentiviral

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pIC247

   Plasmid

   #69741

   Purpose

   Retroviral expression of Borealin

   Depositor

   Iain Cheeseman

   Insert

   Borealin (CDCA8 Human)
   

   Use

   Retroviral

   Tags

   eYFP-TEV-S

   Expression

   Mammalian

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

7. TFORF2950

   Plasmid

   #144426

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MAFB (MAFB Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-ABL1

   Plasmid

   #23939

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ABL1 (ABL1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVpuro-eGFP-hcGAS

   Plasmid

   #108674

   Purpose

   For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

   Insert

   eGFP-cGAS (CGAS Human)
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. CL20_mEGFP_EEF2_SMARCA4

    Plasmid

    #205808

    Purpose

    Express mEGFP-tagged fusion protein, EEF2_SMARCA4 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EEF2_SMARCA4 (EEF2, SMARCA4 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Kif5b-Myc

    Plasmid

    #186614

    Purpose

    Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B fused to Myc tag

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Heavy Chain isoform 5b (KIF5B Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFP-Parkin-IRES-zeo

    Plasmid

    #61728

    Purpose

    Expression of human Parkin fused to eYFP

    Depositor

    Douglas Green, Stephen Tait

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Tags

    eYFP

    Expression

    Mammalian

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. SGTA-EGFP

    Plasmid

    #210373

    Purpose

    Expresses SGTA fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SGTA (SGTA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3414

    Plasmid

    #144890

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NEUROG1 (NEUROG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RBM22

    Plasmid

    #155889

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    RBM22 (RBM22 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-hygro-STING V155M

    Plasmid

    #102599

    Purpose

    Express STING V155M for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    V155M

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPC1-muscle Amphiphysin 2

    Plasmid

    #22213

    Depositor

    Pietro De Camilli

    Article

    Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

    Insert

    Amphiphysin II (BIN1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Myd88-CFP

    Plasmid

    #13026

    Depositor

    Doug Golenbock

    Insert

    myeloid differentiation primary response gene (88) (MYD88 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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