159,557 results

1. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 and H1

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-dual-scafffold-2.0 (pBP49)

   Plasmid

   #218931

   Purpose

   The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Insert

   dual scaffolds for CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.CB7.CI.mCerulean.WPRE.RBG

   Plasmid

   #105557

   Purpose

   AAV expression of mCerulean from CB7 promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1 and AAV9

   Insert

   mCerulean
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CB7

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C3-PLK4-3xFLAG

   Plasmid

   #69837

   Purpose

   This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Use

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT-ChemoG-ATP

   Plasmid

   #193818

   Purpose

   CMV overexpression of ChemoG-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTKLP-tetA

   Plasmid

   #71325

   Purpose

   tetA landing pad template plasmid

   Depositor

   Thomas Kuhlman

   Article

   Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSSmKate2-N1

   Plasmid

   #31867

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-MRLC T18A,S19A

   Plasmid

   #35681

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T18A and S19A

   Promoter

   CMV

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. CXCR2-DuET

    Plasmid

    #213219

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR2 (CXCR2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMK287 (mAID-Hygro)

    Plasmid

    #72825

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Hygro
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-SKP1-T131A

    Plasmid

    #213482

    Purpose

    Expresses human SKP1 T131A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

    Insert

    human Flag tagged SKP1-T131A (SKP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-Zeo

    Plasmid

    #75088

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-Halo

    Plasmid

    #200197

    Purpose

    Expression for SV40 NLS

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    3X SV40 NLS-Halo
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 . 11():63.

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRBV19

    Plasmid

    #123453

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV19 (TRBV19 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. BirA-Myc_N_ETS1

    Plasmid

    #167700

    Purpose

    Expresses ETS1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETS1 (ETS1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-CD63-L7Ae

    Plasmid

    #180503

    Purpose

    Human CD63-L7Ae fusion protein

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Human CD63-L7Ae fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. phage-ubc-nls-ha-tdPCP-gfp

    Plasmid

    #40650

    Depositor

    Robert Singer

    Article

    Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

    Insert

    tdPCP-gfp
    

    Use

    Lentiviral

    Tags

    EGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR223-IRAK4

    Plasmid

    #23749

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    IRAK4 (IRAK4 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

22. CIp10 - γmGFP (γmGFP based)

    Plasmid

    #163119

    Purpose

    Expresses in Candida albicans

    Depositor

    Patrick Van Dijck

    Article

    Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

    Insert

    gamma mGFP
    

    Use

    Candida albicans expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. ZsGreen1-N1

    Plasmid

    #54702

    Purpose

    Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    ZsGreen1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pUC19/mChe-Px

    Plasmid

    #183170

    Purpose

    Construct for transient expression of peroxisomes-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Px
    

    Use

    Tags

    10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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26. mCherry-Rab5DN(S34N)

    Plasmid

    #35139

    Depositor

    Sergio Grinstein

    Article

    Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Dominant Negative- S34N

    Promoter

    CMV

    Available since

    March 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1+FLAG-KrascomG12D

    Plasmid

    #206844

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMMP-dnPERK-IRES-eGFP

    Plasmid

    #36954

    Depositor

    Bill Sugden

    Article

    Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

    Insert

    dnPERK (EIF2AK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion of aa592-1061 to create dominant negative

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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