169,843 results

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

No Promoter

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Sos2

Plasmid

#32921

Depositor

Dafna Bar-Sagi

Article

Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

Insert

SOS2 (SOS2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pCbs FlagOmomyc

Plasmid

#113168

Purpose

Expression of FLAG tagged Omomyc in mammalian cells

Depositor

Sergio Nasi

Article

Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

Insert

Omomyc (MYC Human)

Tags

FLAG

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC13N-iCAG.copGFP

Plasmid

#66578

Purpose

CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-MBP-TEV-GG

Plasmid

#215800

Purpose

Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pmCherry-SopF

Plasmid

#135174

Purpose

Expresses an mCherry-SopF fusion in mammalian cells

Depositor

Leigh Knodler

Article

Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

Insert

SopF (STM1239 Salmonella enterica serovar Typhimurium)

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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PP1 7-300

Plasmid

#26566

Depositor

Wolfgang Peti

Article

Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

Insert

PP1a (PPP1CA Human)

Tags

His6, TEV cleavage site, and Thio-6

Expression

Bacterial

Mutation

Contains only aa 7-330

Available Since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmaxRNaseHtrunc

Plasmid

#207858

Purpose

Mammalian expression of PEmaxDRNaseH prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PEmaxDRNaseH

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

M-MLVRTDRNAseHrt

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pKH3-human RSK1

Plasmid

#13841

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK1 (RPS6KA1 Human)

Tags

3x HA

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

PQ5 (cumate-inducible)

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A3_STOP

Plasmid

#161206

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A3 (SLC25A3 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBiFC-bFosVC155

Plasmid

#22013

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bFosVC155

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV4-ApoE3

Plasmid

#87086

Purpose

Expresses ApoE3 in Mammalian cells

Depositor

Bradley Hyman

Article

Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

Insert

Apolipoprotein E3 (APOE Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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Snap-Omp25

Plasmid

#69599

Purpose

Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

Snap-Omp25

Expression

Mammalian

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFlc245

Plasmid

#101021

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSFV3

Plasmid

#92072

Purpose

Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Type

Empty backbone

Use

Empty expression plasmid, for run-off transcripti…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Expression

Mammalian

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-SMC3

Plasmid

#222554

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SMC3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

SMC3 (SMC3 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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GSK3 beta pGEX

Plasmid

#15898

Depositor

Jim Woodgett

Insert

GSK3 beta (GSK3B Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 1, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-U6-sgRNA-BFP-Puro

Plasmid

#229014

Purpose

Perturb Seq lentiviral sgRNA vector

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB-wt

Plasmid

#58905

Purpose

mammalian expression of wild type human Rb

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Promoter

CaMKIIa

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-pAceR-Kv2.1PR

Plasmid

#195534

Purpose

Cre-dependent red fluorescent, positive response-polarity voltage indicator; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR-Kv2.1 proximal restriction sequence

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

CAG

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pOpen-T7gene5

Plasmid

#165534

Purpose

DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T7 DNA Polymerase (unmodified)

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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