Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

178,737 results

Showing: 9181 - 9210 of 178737 results

  3. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113193-plasmid-map-sequence-id-216317

    Enlarge

  15. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92120-plasmid-map-sequence-id-197614

    Enlarge

  17. pcDNA3-TrxRFP1

    Plasmid
    #98996
    Purpose
    mammalian expression of a biosensor to monitor thioredoxin redox dynamics
    Depositor
    Huiwang Ai
    Publication
    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
    Tags
    His-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98996-plasmid-map-sequence-id-190084

    Enlarge

  19. AICSDP-35:AAVS1-mEGFP

    Plasmid
    #91565
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    91565-plasmid-map-sequence-id-456463

    Enlarge

  25. pCMV-SPORT6-hHMGCR1

    Plasmid
    #86085
    Purpose
    expresses the human HMG-CoA reductase isoform 1
    Depositor
    Anne Galy
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  26. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Publication
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86846-plasmid-map-sequence-id-172845

    Enlarge

  27. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87029-plasmid-map-sequence-id-173001

    Enlarge

  29. pY095

    Plasmid
    #84744
    Purpose
    Expresses huLbCpf1-T2A-GFP and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huLbCpf1
    GFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84744-plasmid-map-sequence-id-196925

    Enlarge

  30. pP{ELAV-GeneSwitch}

    Plasmid
    #83957
    Purpose
    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor
    Depositor
    Haig Keshishian
    Publication
    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98
    Insert
    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    Expression
    Insect
    Promoter
    elav
    Available since
    Dec. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83957-plasmid-map-sequence-id-160516

    Enlarge

Showing: 9181 - 9210 of 178737 results