We narrowed to 10,959 results for: T7
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Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialPromoterT7-LacOAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only
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pTEV5_ RpMatB_2
Plasmid#78206PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingDepositorInsertmatB (His6 C106A S170A R286C Q457C K488A)
TagsHis6ExpressionBacterialMutationC106A S170A R286C Q457C K488APromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
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GFP-V96 ELP
Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorInsertGFP fused to ELP (VPGVG) repeated 96 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
ExpressionMammalianPromoterT7 PromoterAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b
Plasmid#184565PurposeInducible expression of PbuCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Prevotella buccae
ExpressionBacterialPromoterT7 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL1B
Plasmid#67066PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHB-2
Plasmid#140955PurposeFor PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.DepositorInsertphaCAB
ExpressionBacterialMutationR238S mutation in PhaCAvailable SinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
ExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SAE1
Plasmid#175241PurposeBacterial expression and purification, combined with SAE2 to form the E1 enzyme heterodimerDepositorAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLP-pR-himar-apraR
Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
ExpressionBacterialAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
TagsHisExpressionBacterialPromoterT7Available SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d(H189A)-polyA
Plasmid#61554PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertKdm4d (catalytic mutant)
TagsflagExpressionMammalianMutationchanged Histidine 189 (of Kdm4d) to AlaninePromoterCMV and T7Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
TagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-cpLuc2
Plasmid#135629PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)DepositorInsertNAD-cpLuc2 sensor
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
TagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF16
Plasmid#52642Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF16
ExpressionMammalianMutationD431N/D433N/D435N/E442A mutated from GCaMP3PromoterT7Available SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1
Plasmid#184566PurposeInducible expression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
ExpressionBacterialPromoterT7 promoterAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only