4,178 results

1. pICE-RNaseHI-WT-NLS-mCherry

   Plasmid

   #60365

   Purpose

   Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI
   

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA human NEMO

   Plasmid

   #13512

   Depositor

   Kunliang Guan

   Article

   Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

   Insert

   NEMO (IKBKG Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL01009::AtU6p

   Plasmid

   #46968

   Purpose

   Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSERT pcDNA3

   Plasmid

   #15483

   Depositor

   Randy Blakely

   Article

   Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

   Insert

   hSERT (SLC6A4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICE-RNaseHI-D10R-E48R-NLS-mCherry

   Plasmid

   #60367

   Purpose

   Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Insert

   RNase HI-D10R-E48R
   

   Tags

   NLS from SV40 T antigen and mCherry

   Expression

   Mammalian

   Mutation

   D10R+E48R; catalyticaly inactive

   Promoter

   CMV-tet

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6/V5/DEST-VPS35

   Plasmid

   #21691

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Lentiviral; Invitrogen gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 hygro shRNA E-cadherin

   Plasmid

   #18802

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   E-cadherin shRNA (CDH1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. V245 pCEP-4HA B56alpha

   Plasmid

   #14532

   Depositor

   David Virshup

   Article

   Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

   Insert

   PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)
   

   Tags

   4 HA

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

    Plasmid

    #49771

    Purpose

    Level 1 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    35Sp::hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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