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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,239 results for: membrane gfp

Showing: 1201 - 1239 of 1239 results

  13. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234873-plasmid-map-sequence-id-469050

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  14. ORAI1-YFP V107M-L273D-L276D

    Plasmid
    #114183
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M/L273D/L276D
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114183-plasmid-map-sequence-id-218685

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  15. ORAI1-YFP T184M-L273D-L276D

    Plasmid
    #114184
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M/L273D/L276D
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114184-plasmid-map-sequence-id-220961

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  27. NK85

    Plasmid
    #176611
    Purpose
    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.
    Depositor
    Meghan Morrissey
    Article
    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1
    Insert
    FCERG
    Use
    Lentiviral
    Tags
    EGFP and HA, SNAP-tag
    Expression
    Mammalian
    Mutation
    C25A
    Promoter
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176611-plasmid-map-sequence-id-362592

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  29. MLKS2delTMexp

    Plasmid
    #130863
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of transmembrane domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130863-plasmid-map-sequence-id-255916

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  31. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

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  32. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Use
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Promoter
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

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  35. KCC2-pHext

    Plasmid
    #104076
    Purpose
    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)
    Depositor
    Igor Medyna
    Article
    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00
    Insert
    KCC2 (b isoform) (Slc12a5 Rat)
    Use
    Tags
    pHluorine tag inserted in the second putative tra…
    Expression
    Mammalian
    Mutation
    EcoR1 site in rat KCC2 was removed by silent muta…
    Promoter
    UbC
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104076-plasmid-map-sequence-id-199360

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Showing: 1201 - 1239 of 1239 results