We narrowed to 9,448 results for: Coli

1. pET28a-MinE-His

   Plasmid

   #133623

   Purpose

   Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

   Insert

   MinE-His (minE E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a isPETase

   Plasmid

   #225759

   Purpose

   Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

   Depositor

   Jason Boock

   Article

   Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

   Insert

   isPETase
   

   Tags

   6xHis Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-Trx-mSA2

   Plasmid

   #52320

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Tags

   FLAG, His, S tag, TEV site, and Thioredoxin

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMAH-EcaYRS

   Plasmid

   #153558

   Purpose

   Amber suppression for incorporating 3-aminotyrosine to proteins in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

   Inserts

   EcaYRS
   amber suppression tRNA (multiple copies)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD24-sfGFPx2

   Plasmid

   #51559

   Purpose

   Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)

   Depositor

   Francisco Malagon, Sankar Adhya

   Article

   Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

   Insert

   superfolder GFP
   

   Tags

   GLESTCRHASLAVLADERRFSA and MARARA

   Expression

   Bacterial

   Mutation

   Contains additonal aa in the N and C terminus (in…

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB1-spect-PthrC3_8-eGFP

   Plasmid

   #107411

   Purpose

   This plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

   Depositor

   Kang Zhou

   Article

   Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   PthrC3_8

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXD70LacZ1-promoterless-LacZ

   Plasmid

   #191617

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Erick Strauss, Tadhg Begley

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Erick Strauss, Tadhg Begley

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBTR(hCc)

    Plasmid

    #61026

    Purpose

    Expresses horse holocytochrome c in E. coli

    Depositor

    Gary Pielak

    Article

    Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

    Insert

    horse cytochrome c (CYCS Equus*)
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    *see comments

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pSW068

    Plasmid

    #140036

    Purpose

    KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Expression

    Bacterial

    Promoter

    cLac143

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA24N_6xHis-TEV-rpsA

    Plasmid

    #83585

    Purpose

    Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

    Depositor

    Nils Walter

    Article

    Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

    Insert

    rpsA (rpsA Escherichia coli)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    p-T5 Lac

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5 HA3-eDHFR-T2A-puro

    Plasmid

    #86395

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A puro
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A puro

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKL004

    Plasmid

    #98920

    Purpose

    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    GCaMP6f tethered to mRuby
    

    Tags

    His and mRuby3

    Expression

    Bacterial

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. EcMBP165-cpGFP.PPYF.T203V.pRSET

    Plasmid

    #33373

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    Improved MBP based Maltose Biosensor
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30del

    Plasmid

    #106902

    Purpose

    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5 HA3-eDHFR-T2A-neo

    Plasmid

    #86396

    Purpose

    for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

    Depositor

    David Bentley

    Article

    Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

    Insert

    HA3 eDHFR T2A neo
    

    Use

    CRISPR

    Tags

    HA3 eDHFR T2A neo

    Mutation

    R12Y, G67S and Y100I mutations in E coli DHFR

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a-MinC

    Plasmid

    #133628

    Purpose

    Expression vector for the Escherichia coli MinC with an N-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Loose et al Nat Struct Mol Biol. 2011 May;18(5):577-

    Insert

    His-MinC (minC E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLOXp-GUS

    Plasmid

    #154059

    Purpose

    Level 0.5 Golden Gate vector, loxP-GUS-nosT-loxP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    beta-glucoronidase (E. coli)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMB1-spect-PthrC-eGFP

    Plasmid

    #107408

    Purpose

    This plasmid contain promoter PthrC, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PthrC

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSZT025

    Plasmid

    #140033

    Purpose

    KO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Expression

    Bacterial

    Promoter

    cLac143

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOVC1_EcSSPB-nano_X

    Plasmid

    #131212

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET30a-YedK

    Plasmid

    #86884

    Purpose

    Bacterial expression of E. coli SRAP ortholog YedK

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK (yedK Eschericia coli)
    

    Tags

    6xHis, HA tag, and S-Tag

    Expression

    Bacterial

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pASKt15C+SrtA

    Plasmid

    #64983

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK667

    Plasmid

    #71690

    Purpose

    Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

    Insert

    acetate kinase (ackA Escherichia coli)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMB1-spect-PthrC3-eGFP

    Plasmid

    #107409

    Purpose

    This plasmid contain promoter PthrC3, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PthrC3

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSG 413

    Plasmid

    #63859

    Purpose

    Detect translation initiation from CUG start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    CUG start codon

    Promoter

    PtacI

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pMH-HT_ygiF-FL

    Plasmid

    #127795

    Purpose

    Expresses full-length ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSG 417

    Plasmid

    #63864

    Purpose

    Detect translation initiation from AUU start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    AUU start codon

    Promoter

    PtacI

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pSG 416

    Plasmid

    #63863

    Purpose

    Detect translation initiation from AUA start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    AUA start codon

    Promoter

    PtacI

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

34. EC.RNe.TYB1.a1-400.wt

    Plasmid

    #17949

    Depositor

    David McKay

    Article

    Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

    Insert

    Escherichia coli RNase E residues 1-400
    

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    residues 1-400

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pANY2

    Plasmid

    #112196

    Purpose

    This plasmid is used for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

    Type

    Empty backbone

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMB1-spect-PthrC4-eGFP

    Plasmid

    #107410

    Purpose

    This plasmid contain promoter PthrC4, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

    Depositor

    Kang Zhou

    Article

    Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

    Insert

    eGFP
    

    Expression

    Bacterial

    Promoter

    PthrC4

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pASKt15C+SrtA2

    Plasmid

    #64984

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSG 415

    Plasmid

    #63862

    Purpose

    Detect translation initiation from AUC start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    AUC start codon

    Promoter

    PtacI

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pSG 414

    Plasmid

    #63861

    Purpose

    Detect translation initiation from UUG start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    UUG start codon

    Promoter

    PtacI

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pSG 431

    Plasmid

    #63866

    Purpose

    Detect translation initiation from GUG start codon (E. coli lacZ)

    Depositor

    Albert Dahlberg

    Article

    O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

    Insert

    lacZ (lacZ Escherichia coli)
    

    Expression

    Bacterial

    Mutation

    GUG start codon

    Promoter

    PtacI

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

41. EC.RNe.TYB1.a1-395.wt

    Plasmid

    #17950

    Depositor

    David McKay

    Article

    Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

    Insert

    Escherichia coli RNase E residues 1-395
    

    Tags

    intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    residues 1-395

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

42. pHL1575

    Plasmid

    #53028

    Purpose

    PconNoHind:csrB, PLtetO-1:RBS(st3):csrD

    Depositor

    Han Lim

    Article

    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

    Inserts

    csrB
    csrD
    

    Use

    Synthetic Biology

    Tags

    RBS (st3)

    Expression

    Bacterial

    Promoter

    PconNoHind and pLtetO-1

    Available Since

    May 14, 2014

    Availability

    Academic Institutions and Nonprofits only

43. LSAM domain expression plasmid

    Plasmid

    #240215

    Purpose

    Plasmid for the recombinant expression of the LSAM domain of human legumain in E. coli.

    Depositor

    Elfriede Dall

    Article

    Dahms et al STAR Protocols, 6, 3, 103991, 19 Septemb

    Insert

    LSAM (LGMN Human)
    

    Tags

    PC1pro

    Expression

    Bacterial

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSIG1

    Plasmid

    #226908

    Purpose

    MoClo-compatible vectors for dsRNA production in E. coli

    Depositor

    Li Hung Chen

    Article

    Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSIG2

    Plasmid

    #226909

    Purpose

    MoClo-compatible vectors for dsRNA production in E. coli

    Depositor

    Li Hung Chen

    Article

    Wu et al Plant Methods. 2025 Jul 11;21(1):96. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLN34 - pBait-5GC[1xMS2]-McaS

    Plasmid

    #222403

    Purpose

    arabinose-induced synthesis of a hybrid RNA with an MS2 hairpin surrounded by a 5bp GC clamp with E coli McaS inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.

    Depositor

    Katherine Berry

    Article

    Linh D. Nguyen et al Sci Rep 15, 3820 (2025)

    Insert

    McaS (mcaS E. coli)
    

    Tags

    1xMS2 hairpin, 5GC clamp

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pkk223_EcMutY

    Plasmid

    #213110

    Purpose

    pkk223 with E coli MutY insert

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Insert

    MutY (adenine glycosylase) (mutY Escherichia coli)
    

    Expression

    Bacterial

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pOVC1_X_EcSSPB-micro

    Plasmid

    #131218

    Purpose

    Expresses EcSSPB-nano (Escherichia coli) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-micro
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pOVC1_X_EcSSPB-nano

    Plasmid

    #131219

    Purpose

    Expresses EcSSPB-micro (Escherichia coli) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-nano
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pOVC1_EcSSPB-micro_X

    Plasmid

    #131213

    Purpose

    Expresses EcSSPB-micro (Escherichia coli) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    EcSSPB-micro
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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