Skip to main content
Addgene
Showing: 1501 - 1550 of 9150 results

  1. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

    Enlarge

  3. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid
    #29655
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, GST, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29655-plasmid-map-sequence-id-196920

    Enlarge

  4. pH6-MBP-TEV-BirA

    Plasmid
    #179694
    Purpose
    Expresses His6 tagged MBP-BirA fusion protein
    Depositor
    Mark Arbing
    Insert
    BirA
    Use
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179694-plasmid-map-sequence-id-352195

    Enlarge

  5. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid
    #29657
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, NusA, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29657-plasmid-map-sequence-id-418922

    Enlarge

  14. pColdII-SIRPT1-Xenopus (Silurana) tropicalis

    Plasmid
    #75355
    Purpose
    Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376
    Depositor
    Kalle Gehring
    Insert
    Sacsin Xenopus (Silurana) tropicalis 87-1376
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75355-plasmid-map-sequence-id-142888

    Enlarge

  16. pet15_rbsk

    Plasmid
    #220227
    Purpose
    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    ribokinase (rbsK E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220227-plasmid-map-sequence-id-436827

    Enlarge

  18. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

    Enlarge

  19. pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)

    Plasmid
    #30185
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), FlAsH, MBP, and TEV cleav…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    30185-plasmid-map-sequence-id-13873

    Enlarge

  21. pGEX4T1-WWP1F792D

    Plasmid
    #179328
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1 F792D
    Depositor
    Arthur Haas
    Insert
    WWP1 F792D (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    F792D mutation; Codon Optimized for expression in…
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179328-plasmid-map-sequence-id-358707

    Enlarge

  22. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

    Enlarge

  25. pTW386b

    Plasmid
    #237320
    Purpose
    expresses O. sativa Cpn60α Cpn60β Cpn20-1 Raf1 Raf2 RbcX Bsd2
    Depositor
    Tina Wang
    Insert
    O. sativa Cpn60α Cpn60β Cpn20-1 Raf1 Raf2 RbcX Bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    237320-plasmid-map-sequence-id-470243

    Enlarge

  26. PgyrA_gfp

    Plasmid
    #212146
    Purpose
    Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212146-plasmid-map-sequence-id-420759

    Enlarge

  27. pHflu1

    Plasmid
    #203878
    Purpose
    pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer
    Depositor
    Bogumil Karas
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    203878-plasmid-map-sequence-id-408184

    Enlarge

  28. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177211-plasmid-map-sequence-id-349887

    Enlarge

  29. pLNKR1

    Plasmid
    #67968
    Purpose
    High copy number plasmid encoding part of the tra Operon PY-traR
    Depositor
    Joshua Leonard
    Insert
    tra Operon (First Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67968-plasmid-map-sequence-id-121285

    Enlarge

  30. pLNKR2

    Plasmid
    #67969
    Purpose
    High copy number plasmid encoding part of the tra Operon traC-artA
    Depositor
    Joshua Leonard
    Insert
    tra Operon (second Quarter)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67969-plasmid-map-sequence-id-121291

    Enlarge

  31. pCas9CR5

    Plasmid
    #120227
    Purpose
    pCas9CR4 with stop codon to prevent translation of ssrA degradation tag
    Depositor
    Christopher Reisch
    Insert
    SpCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Stop codon inserted before ssrA degradation tag
    Promoter
    pTet
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120227-plasmid-map-sequence-id-233288

    Enlarge

  32. pColdII-SIRPT1-Ceratotherium-simum-simum

    Plasmid
    #78111
    Purpose
    Nt His-tag Ceratotherium-simum-simum Sacsin 89-1366
    Depositor
    Kalle Gehring
    Insert
    Sacsin Ceratotherium-simum-simum 89-1366
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78111-plasmid-map-sequence-id-145661

    Enlarge

  33. pColdII-SIRPT1-Mustela putorius furo

    Plasmid
    #78116
    Purpose
    Nt His-tag Mustela putorius furo Sacsin 94-1381
    Depositor
    Kalle Gehring
    Insert
    Sacsin Mustela putorius furo 94-1381
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78116-plasmid-map-sequence-id-145629

    Enlarge

  34. pHL1726

    Plasmid
    #53032
    Purpose
    PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and hfqlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pBDC

    Plasmid
    #135188
    Purpose
    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    CmR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135188-plasmid-map-sequence-id-273773

    Enlarge

  47. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

    Enlarge

Showing: 1501 - 1550 of 9150 results