We narrowed to 15,231 results for: LEA

1. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

   Plasmid

   #124494

   Purpose

   Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Caspase-1 cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

   Plasmid

   #79383

   Purpose

   Cas9 gRNA variant to recruit ms2 proteins

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   Zhang 2.0 + scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V

   Plasmid

   #162618

   Purpose

   Allows for transcription of mCerulean fluorophore half fused to mutant TDP-43-M337V for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M337V

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124431

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Zebrafish (if expression level is too high in zeb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

   Plasmid

   #67648

   Purpose

   I-SceI cleavage site at lys1-hphMX6

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   lys1 (lys1 Fission Yeast)
   hphMX6
   

   Use

   To test i-scei cleavage efficiency

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

   Plasmid

   #180468

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   KRAS4A (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   V5-mScarlet-I

   Expression

   Mutation

   Promoter

   kdrl

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

   Plasmid

   #126025

   Purpose

   express RFP- and mCer-tagged LARIAT for optogenetic clustering

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

    Plasmid

    #127327

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

    Plasmid

    #127326

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

    Plasmid

    #79380

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)

    Plasmid

    #70744

    Purpose

    for building 3-color reporter plasmid

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    Cerulean-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

    Plasmid

    #59827

    Purpose

    Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera1

    Plasmid

    #79379

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera4

    Plasmid

    #79382

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Chimera 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

    Plasmid

    #24334

    Depositor

    Didier Stainier

    Article

    Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

    Insert

    H2B
    

    Use

    Cre/Lox

    Tags

    GFP

    Expression

    Worm

    Mutation

    mCherry driven by an HSP70 promoterCerulean drive…

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved

    Plasmid

    #231215

    Purpose

    Cleaved-myc-IL-10

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    c-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N36I, N110I, K117N, F129L

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

    Plasmid

    #204811

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,

    Plasmid

    #110201

    Purpose

    Melanocyte-specific expression of the LexPR-2A-Cerulean fusion

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    LexPR transactivator - 2A - Cerulean
    

    Use

    Tags

    Cerulean

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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