We narrowed to 12,214 results for: LEA

Showing: 601 - 620 of 12214 results

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pNCS mCherry

Plasmid

#91769

Purpose

Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

Depositor

Erik Rodriguez, Roger Tsien

Publication

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Insert

monomeric Cherry

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

See Depositor Comments

Promoter

T7

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET StrepII TEV LIC cloning vector (1R)

Plasmid

#29664

Depositor

Scott Gradia

Type

Empty backbone

Tags

Strep II and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCRISPRx-Sth1Cas9-L5

Plasmid

#140993

Purpose

Sth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in Mycobacteria

Depositor

Wilbert Bitter

Publication

Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

Inserts

Sth1Cas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified; Integrative in mycobactreia

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Publication

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Publication

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 TFAM-mClover

Plasmid

#129574

Purpose

Expression of mClover fused TFAM

Depositor

Stephen Tait

Publication

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSV-PGC1

Plasmid

#3

Depositor

Bruce Spiegelman

Publication

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Expression

Mammalian

Available since

May 23, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Publication

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Publication

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Thy1-Brainbow-1.1 M

Plasmid

#18726

Depositor

Joshua Sanes

Publication

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Thy1-Brainbow-2.1 R

Plasmid

#18727

Depositor

Joshua Sanes

Publication

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Publication

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMJ824

Plasmid

#39314

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Publication

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SENP2 (SENP2 Human)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMT413

Plasmid

#8606

Depositor

Robert Hartley

Publication

Hartley et al J Mol Biol 1988 Aug 20;202(4):913-5.

Insert

barnase, barstar

Tags

phoA signal sequence to barnase

Expression

Bacterial

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pAdSh.PGK.Cas9

Plasmid

#58253

Purpose

Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

Depositor

Manuel Goncalves

Publication

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

Human codon-optimized S. pyogenes Cas9

Use

Adenoviral and CRISPR

Expression

Mammalian

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3 luciferase

Plasmid

#26612

Depositor

Jie Chen

Publication

Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

firefly luciferase

Expression

Mammalian

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6P1-3xMBT

Plasmid

#46987

Purpose

Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

Depositor

Or Gozani

Publication

Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

Insert

Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)

Tags

GST and PreScission cleavage site

Expression

Bacterial

Mutation

contains amino acids 190–530 of accession NP_0562…

Promoter

tac

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRCC-N

Plasmid

#81192

Purpose

Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

Depositor

Eckhard Boles

Publication

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below. and WT - cod…

Promoter

ROX3 and SNP52p

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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