We narrowed to 15,613 results for: grna
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Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialPromoterT7 (x2)Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterhuman U6 promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-Cas9
Plasmid#96924Purposefor Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111DepositorInsertCas9
UseLentiviralTags3xFlag and V5ExpressionMammalianPromoterEF1aAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W
Plasmid#67985PurposeCas9 activity reporter (control) with mCherry and BFPDepositorInsertU6gRNA cassette, PGKmCherryABFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60227PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
vHB16
Plasmid#193455PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInserttRNA-gRNA
UseCRISPRPromoterU6Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas34
Plasmid#82386PurposesgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
PromoterPEZ3Available SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1
Plasmid#138460Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRABDepositorInsertMCP-KRAB-IRES-zsGreen1
UseCRISPR and LentiviralExpressionMammalianPromoterEF1AAvailable SinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralPromoterhUBC promoterAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-mCherry-TUBB
Plasmid#66942PurposeCRISPaint target selector TUBBDepositorInsertgRNA TUBB
Available SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-mCherry-ACTG1
Plasmid#66943PurposeCRISPaint target selector ACTG1DepositorInsertgRNA ACTG1
Available SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
B52 (empty plasmid backbone to express 2 sgRNAs)
Plasmid#100708PurposeEmpty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR_DKO
Plasmid#183192PurposePlasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterhU6, mU6Available SinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
Plasmid#154003PurposeVector expressed CasRx and sgRNA_LACZ for AAV packageDepositorInsertCasRx
UseAAV and CRISPRPromoterEFSAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSOX11.1.0-gDNA
Plasmid#176378PurposeExpresses spCas9 and gRNA sequence targeting SOX11DepositorInsertSOX11 gRNA
UseCRISPRAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBACH2.2.0-gDNA
Plasmid#176375PurposeExpresses spCas9 and gRNA sequence targeting BACH2DepositorInsertBACH2 gRNA
UseCRISPRAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-Sirius-8XPP7
Plasmid#121943PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianPromotermouse U6 promoterAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p104_gRNA_Sp_CD3e
Plasmid#213780PurposeExpresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.DepositorInsertSp gRNA
UseCRISPR and LentiviralAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRExpressionYeastPromoterSNR52Available SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only