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We narrowed to 59,655 results for: plasmid

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Showing: 601 - 620 of 59655 results

  1. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90085-plasmid-map-sequence-id-182691

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  3. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid
    #60362
    Purpose
    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FUS (FUS Human)
    Tags
    FLAG and GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60362-plasmid-map-sequence-id-356956

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  6. EGFP-CAAX

    Plasmid
    #86056
    Purpose
    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens Harvey rat sarcoma (HRAS Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains the region from 825-857 of HRAS as a far…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86056-plasmid-map-sequence-id-177924

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  8. pGL3-NFAT luciferase

    Plasmid
    #17870
    Purpose
    3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
    Depositor
    Jerry Crabtree
    Publication
    Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3x NFAT binding sequence (IL2 )
    Use
    Luciferase
    Tags
    IL2 promoter and Luciferase
    Expression
    Mammalian
    Promoter
    IL-2 promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    17870-plasmid-map-sequence-id-520532

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  10. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Publication
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104992-plasmid-map-sequence-id-213507

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  11. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112203-plasmid-map-sequence-id-473446

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  12. pX330A-nBRD4/PITCh

    Plasmid
    #91794
    Purpose
    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chicken β-actin promoter
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91794-plasmid-map-sequence-id-429087

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  14. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

    Plasmid
    #44361
    Purpose
    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Publication
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV; Adeno associated viral vector
    Tags
    mCherry
    Promoter
    human Synapsin 1
    Available since
    May 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    44361-plasmid-map-sequence-id-148598

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  16. pAnc80L65

    Plasmid
    #68837
    Purpose
    Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details
    Depositor
    Luk Vandenberghe
    Publication
    Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1
    Insert
    Ancestral AAV Capsid Anc80L65
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Promoter
    Rep2
    Available since
    Nov. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68837-plasmid-map-sequence-id-121219

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  18. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid
    #50476
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50476-plasmid-map-sequence-id-171073

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Showing: 601 - 620 of 59655 results