We narrowed to 56,851 results for: plasmid
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Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
phDLX1-N174
Plasmid#60859Purpose2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoterDepositorAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRMC2
Plasmid#68940Purposetetracycline-inducible expression vector for Staphylococcus aureusDepositorTypeEmpty backboneUseTet inducible e. coli/staphylococcus shuttle vect…ExpressionBacterialAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2
Plasmid#17218PurposeRetroviral expression of human SOX2DepositorAvailable SinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cre Stoplight 2.4
Plasmid#37402PurposeCre activity reporter plasmid; Zsgreen is expressed in cells without Cre, mCherry is expressed with CreDepositorInsertCre Stoplight 2.4
TagsZsGreen1 and mCherryExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTP1174
Plasmid#104162PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)DepositorInsertAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-HA
Plasmid#141320PurposePlasmid to carry out IVT of RfxCas13d (human codon-optimized)DepositorInsertRfx-Cas13d
UseCRISPRTagsHAAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
TagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialAvailable SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro LEF1
Plasmid#27023DepositorAvailable SinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianPromoterTight TRE promoterAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-Guide
Plasmid#85401PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase-W159H-S238F
Plasmid#112203PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
Tags6XHISExpressionBacterialMutationW159H, S238FPromoterN/AAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Klf4
Plasmid#13370PurposeRetroviral expression of mouse Klf4DepositorAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
TagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA neo
Plasmid#104992PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF1389
Plasmid#104962PurposeBacterial expression plasmid of bdSENP1 proteaseDepositorInsertSENP1
TagsHis14 tag and Tev protease cleavage siteExpressionBacterialPromoterlacUV5Available SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ203 (pMDC32-Ubi1)
Plasmid#86207PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterMaize ubiquitin 1 (ZmUbi1)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only