159,557 results

1. pRSET-6xTR-TUBE

   Plasmid

   #110313

   Purpose

   E. coli expression and purification of 6xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   6xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-ALK5 wt

   Plasmid

   #80876

   Purpose

   mammalian expression of ALK5 WT

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET His6 TEV LIC cloning vector (1B)

   Plasmid

   #29653

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRLSIN.cPPT.PGK-GFP.WPRE

   Plasmid

   #12252

   Purpose

   3rd generation lentiviral backbone

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Bx

   Plasmid

   #51552

   Purpose

   Expresses Bxb1 integrase in mammalian cells.

   Depositor

   Michele Calos

   Article

   Zhu et al Nucleic Acids Res. 2013 Dec 4.

   Insert

   Bxb1 integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCherry.90 alpha

   Plasmid

   #108222

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 alpha

   Depositor

   Didier Picard

   Article

   Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

   Insert

   mCherry-Hsp90a (HSP90AA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5FRT/Flag-FLJ10081

   Plasmid

   #64775

   Purpose

   Mammalian expression of Flag-tagged FLJ10081 (NSL3)

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   FLJ10081 (KANSL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCasKP-hph

   Plasmid

   #117232

   Purpose

   Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACM4

    Plasmid

    #49797

    Purpose

    ePathBrick vector for pathway engineering in E. coli.

    Depositor

    Mattheos Koffas

    Article

    Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

    Type

    Empty backbone

    Use

    Tags

    S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-EGFP-Cdc42(wt)

    Plasmid

    #12599

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (WT) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234452

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. fluoPEER-GFP>BFP

    Plasmid

    #185477

    Purpose

    Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP-Y66-editing-site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN-sacB

    Plasmid

    #196249

    Purpose

    Plasmid containing the sacB gene

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attR2-sacB-attL3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    sacB promoter

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGR420

    Plasmid

    #213782

    Purpose

    Integrates onto p1 a partial LEU2 sequence to be subsequently used as an integration flank

    Depositor

    Chang Liu

    Insert

    MET15
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20_EGFP

    Plasmid

    #228878

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-neo:Beta2meGFP

    Plasmid

    #45096

    Depositor

    Henry Lester

    Article

    Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

    Insert

    nAChR beta2 (Chrnb2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    There are strain differences between C57BJ/6 and …

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge