162,109 results

1. pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

   Plasmid

   #187916

   Purpose

   Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   polymerase basic protein 2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO human shRNA 1 DEPTOR

   Plasmid

   #21335

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   DEPTOR shRNA 1 (DEPTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pTetO-hOSK-CASH-1

   Plasmid

   #188977

   Purpose

   A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   Doxycycline inducible reprogramming donor cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.Syn.NES-jRGECO1b.WPRE.SV40

   Plasmid

   #100857

   Purpose

   AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1b
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB-gDA3m-IRES-mCherry-CAAX

   Plasmid

   #208692

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_gDA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Cdc42-2G

   Plasmid

   #68813

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Lentiviral

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK503

   Plasmid

   #110616

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGEX-Bcatfl

   Plasmid

   #24193

   Depositor

   David Rimm

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223-AURKA

   Plasmid

   #23532

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   AURKA (AURKA Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   I57V

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pINDUCER21-RORA

    Plasmid

    #51303

    Purpose

    Expression of RORA after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    RAR-related orphan receptor A (RORA Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Expression

    Mutation

    full length ORF

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. tdTomato-VE-Cadherin-N-10

    Plasmid

    #58142

    Purpose

    Localization: Tight Junctions, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    VE-Cadherin (CDH5 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    I503T in VE-Cadherin

    Promoter

    CMV

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCPP3373

    Plasmid

    #128718

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopQ1-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous point mutation in the wobble position…

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GPR120-DuET

    Plasmid

    #213261

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR120 (FFAR4 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCVhygro-F-G9a

    Plasmid

    #41721

    Purpose

    Retroviral expression of human G9a in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9a (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. ClpP-his

    Plasmid

    #22144

    Depositor

    Robert Sauer

    Article

    Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

    Insert

    ClpP-His6
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    C-terminal His tag

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBoBi-hGOLGA2-C-PEN2

    Plasmid

    #183649

    Purpose

    Lentiviral expression of a golgi-residential PEN2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 and GOLGA2 (PSENEN, GOLGA2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-NHERF1-FL

    Plasmid

    #28291

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF1 (NHERF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY1173_ADAR1p150

    Plasmid

    #193192

    Purpose

    Express full length ADAR1p150

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    ADAR1p150 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TetON-Bmpr1A-ca

    Plasmid

    #217536

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    constitutively active BMP receptor 1a (BMPR1A Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet operon (TetO)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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