We narrowed to 13,962 results for: FLU;

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mRuby2-Lysosomes-20

Plasmid

#55902

Purpose

Localization: Lysosome Membrane, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LAMP1

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise2-LaminB

Plasmid

#98830

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

LaminB

Tags

mTurquoise2

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCyterm-mScarlet3_N1

Plasmid

#189778

Purpose

Construct for testing oligimerization of mScarlet3 in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra2

Plasmid

#179475

Purpose

Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra2
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMito-dCherry-FRB

Plasmid

#186573

Purpose

Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

Dark MitoTrap

Expression

Mammalian

Mutation

Mutation of "K70N" to render mCherry no…

Promoter

CMV

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-tdKatushka2-WPRE

Plasmid

#193337

Purpose

Expresses tdKatushka2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tdKatushka2

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCalN-ddGFP-A

Plasmid

#40290

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

Calnexin (CANX Human)

Tags

ddGFP-A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

Plasmid

#170048

Purpose

Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA

Plasmid

#108236

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter

Expression

Bacterial

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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ZJOM153

Plasmid

#133642

Purpose

Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

Vph1-2GFP (VPH1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3

Plasmid

#209727

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-mKate2

Plasmid

#104009

Purpose

Plasmid encoding mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mKate2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, V45A, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-GFP

Plasmid

#86537

Purpose

Retrovirus expressing GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

GFP

Use

Retroviral

Expression

Mammalian

Promoter

Synapsin I (human)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEDA5_GFPmut3_Y66H

Plasmid

#80085

Purpose

Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

Depositor

Timothy Whitehead

Article

Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

Insert

GFPmut3_Y66H

Tags

6x-His tag

Expression

Bacterial

Mutation

changed Tyrosine 66 to Histidine

Promoter

proB

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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