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171,784 results

Showing: 9331 - 9360 of 171784 results

  3. pLenti-CMV-PtenWT-Puro

    Plasmid
    #135676
    Purpose
    Constitutive expression (cMV promoter) of wildtype Pten cDNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    Pten (Pten Mouse)
    Use
    Lentiviral
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    135676-plasmid-map-sequence-id-327583

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  6. mCherry-TGNP-N-10

    Plasmid
    #55145
    Purpose
    Localization: Golgi Complex, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    TGNP (TGOLN2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55145-plasmid-map-sequence-id-339930

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  8. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

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  11. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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  12. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid
    #178330
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn iGluSnFR3 v857.SGZ
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…
    Expression
    Mammalian
    Available Since
    Feb. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    178330-plasmid-map-sequence-id-353668

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  19. SUV[SET]-dCas9

    Plasmid
    #100088
    Purpose
    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    SUVAR39H1 (SUV39H1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-SUV[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    deleted aa 1-76
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100088-plasmid-map-sequence-id-197559

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  20. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

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  27. CHRM2-DuET

    Plasmid
    #213208
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM2 (CHRM2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213208-plasmid-map-sequence-id-430770

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Showing: 9331 - 9360 of 171784 results