We narrowed to 12,235 results for: HAL

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pMTKM30

Plasmid

#233502

Purpose

Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

TDH3-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM31

Plasmid

#233503

Purpose

Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Insert

PGK1-tetO2p

Use

Synthetic Biology

Expression

Yeast

Mutation

addition of two tetracycline operator sequeneces

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-92)

Plasmid

#37307

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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FAB(ER-mito)

Plasmid

#214427

Purpose

Detection of ER-mito contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST10-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2PHR

Plasmid

#28244

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

cry2 (CRY2 Mustard Weed)

Tags

Gal4BD

Expression

Yeast

Available Since

March 23, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:CIBN

Plasmid

#135986

Purpose

Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the CIBN domain

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR

Plasmid

#188336

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hyg-AtAID:HA (p5339)

Plasmid

#239363

Purpose

pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Arabidopsis Auxin Inducible Degradation domain

Use

Bacterial

Tags

1 x HA epitope

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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Luc-noPS

Plasmid

#177940

Purpose

Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

Luc2

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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SP6-VEE-IRES-Puro

Plasmid

#58971

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-control

Plasmid

#238257

Purpose

For overexpression of EGFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEY121

Plasmid

#223468

Purpose

sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-6 (sra-6 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMTKM07

Plasmid

#233479

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pME-loxP-mTagBFP2-stop-pA-loxP

Plasmid

#75158

Purpose

Floxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

loxP-mTagBFP2-stop-pA-loxP

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby2-ZO1-C-14

Plasmid

#55916

Purpose

Localization: Tight Junctions, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

ZO1 (TJP1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PLKO-mcherry-luc-puro-Renilla cDNA

Plasmid

#29783

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

Renilla

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP

Expression

Mammalian

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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