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pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

Plasmid

#189955

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

Plasmid

#105023

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

Plasmid

#105024

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfb (Cobra1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

Plasmid

#105025

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

Plasmid

#105026

Purpose

Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nelfcd (Th1l Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 12

Plasmid

#36109

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 12 (Gng12 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pBRPy CAGGS-Mbd3-IRES-PURO

Plasmid

#52376

Purpose

Mammalian expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Tags

2xFlag (DYKDDK)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pEF‐PIK3IP1

Plasmid

#49214

Purpose

expresses PIK3IP1 in mammalian cells

Depositor

Lawrence Kane

Article

DeFrances et al Eur J Immunol. 2012 Oct;42(10):2754-9. d

Insert

PIK3IP1 (Pik3ip1 Mouse)

Tags

6X-his and myc

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
MyoD-cTAP

Plasmid

#20915

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

MyoD cTAP (Myod1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

TAP tag is fused to the 3' end of the MyoD s…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
KA1870_pGL4-UBCmin-luc2/Esrrb-B'

Plasmid

#124196

Purpose

Luciferase reporter driven by the Esrrb enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (del.Cyto)

Plasmid

#49209

Purpose

expresses Flag tagged mouse Tim3 fragment (no cytosolic domain)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (del.Cyto) (Havcr2 Mouse)

Tags

CD8 signal sequence and FLAG

Expression

Mammalian

Mutation

cytosolic domain deleted, no Tim3 signal sequence…

Promoter

Ef1a

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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Flrt1-AP-His

Plasmid

#71947

Purpose

Expresses the extracellular region of the FLRT1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt1 (Flrt1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Mouse, Human)

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-SV40P-Tet1 enhancer E

Plasmid

#63880

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 enhancer fragment E

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer2

Plasmid

#14777

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pSiP1

Plasmid

#21952

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Expression

Mammalian

Available Since

March 17, 2010

Availability

Academic Institutions and Nonprofits only
HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Reep4-V5

Plasmid

#175120

Purpose

Lentiviral expression of mouse Reep4-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep4 (Reep4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-Actin2-C-18

Plasmid

#54973

Purpose

Localization: Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Alpha-Actin2

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only