We narrowed to 10,337 results for: plasmids 101

1. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ef1a-FAS-GCaMP6f-WPRE

   Plasmid

   #141237

   Purpose

   AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   Human elongation factor-1 alpha (EF-1 alpha)

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

   Plasmid

   #174897

   Purpose

   ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sf GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

   Plasmid

   #174898

   Purpose

   opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TGA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-LS

   Plasmid

   #205438

   Purpose

   Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes

   Depositor

   Masamichi Kamihira

   Article

   Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478

   Inserts

   Gaussia luciferase gene
   spectinomycin resistance gene
   

   Use

   Cre/Lox and Luciferase; Chlamydomonas expression

   Tags

   Expression

   Mutation

   Promoter

   Heat shock protein 70A (HSP70A)/ribulose bisphosp…

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

   Plasmid

   #192132

   Purpose

   Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1-Hsp2Cas9-Y
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Hsp1-Hsp2Cas9 (Y446A)

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABR mCherry-Bcd-LEXY

    Plasmid

    #182596

    Purpose

    Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    LEXY and mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-TAC-OKMS-KRAB-dCas9

    Plasmid

    #120356

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GSX2-P2A-tGFP-DTA

    Plasmid

    #161750

    Purpose

    tGFP plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    turboGFP
    

    Use

    CRISPR

    Tags

    P2A-tGFP

    Expression

    Mammalian

    Mutation

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIW040

    Plasmid

    #134127

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 R236A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIW039

    Plasmid

    #134126

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 and K203A K206A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIW028

    Plasmid

    #134125

    Purpose

    Bacterial expression of human Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-Precission-human Ska1 MTBD (SKA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 132-255 and K183A K184A

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PLKO.1-UPF3B-3'UTR

    Plasmid

    #136044

    Purpose

    UPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRIME-CMV-GFP-shNts2

    Plasmid

    #132714

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Nts

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(2) (Nts Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPRIME-CMV-GFP-shNts1

    Plasmid

    #132713

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shNts(1) (Nts Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

    Plasmid

    #127335

    Purpose

    Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

    Plasmid

    #127309

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge