We narrowed to 4,065 results for: p2a

1. pCMV-SlugCas9-NNG-P2A-puro

   Plasmid

   #214352

   Purpose

   Expresses SlugCas9-NNG and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9-NNG and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiTet-OTX2-P2A-mCherry BlastR

   Plasmid

   #186741

   Purpose

   Dox inducible OTX2-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   OTX2 (OTX2 Human)
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-BE4max-P2A-EGFP (pRZ33)

   Plasmid

   #140083

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with P2A-EGFP

   Depositor

   Keith Joung

   Insert

   Human codon optimized pCAG BE4max with P2A-EGFP
   

   Use

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   D10A (SpCas9)

   Promoter

   CAG

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVH-EF1a-GFP-P2A-KLF4

   Plasmid

   #130694

   Purpose

   Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-Electra2-P2A-EGFP

   Plasmid

   #184936

   Purpose

   Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK348 (BSD-P2A-mAID-mClover)

   Plasmid

   #121182

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   BSD-P2A-mAID-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK437 (mAID-mCherry-P2A-BSD)

   Plasmid

   #168232

   Purpose

   C-terminal tagging with mAID-mCherry

   Depositor

   Masato Kanemaki

   Article

   Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

   Insert

   mAID-mCherry-P2A-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

   Plasmid

   #191457

   Purpose

   Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

   Depositor

   Dorus Gadella

   Article

   van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

   Insert

   G-Ca-FLITS
   

   Use

   Tags

   His-mScarletI-antiFRETlinker-P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK346 (Hygro-P2A-mAID-mCherry)

   Plasmid

   #121180

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

    Plasmid

    #154089

    Purpose

    Improved version to express RUNX1, P2A, ERG in mammalian cells.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-EF1a-AcGFP-P2A-hBRD2

    Plasmid

    #197880

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSC-mCh-P2A-ST-EGFP-CAAX

    Plasmid

    #207636

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amber stop codon at SpyCatcher’s critical lysine

    Promoter

    CMV

    Available since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-emiRFP670-P2A-GFP

    Plasmid

    #197160

    Purpose

    Expresses the protein of emiRFP670-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    emiRFP670-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CAG-EGFP-P2A-FusionRed

    Plasmid

    #191108

    Purpose

    Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    pAAV-CAG-EGFP-P2A-FusionRed
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tet-O-FUW-EGFP-P2A-mCherry

    Plasmid

    #203868

    Purpose

    Tet-inducible lentiviral plasmid expressing EGFP-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK434 (mAID-mClover-P2A-Hygro)

    Plasmid

    #168229

    Purpose

    C-terminal tagging with mAID-mClover

    Depositor

    Masato Kanemaki

    Article

    Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

    Insert

    mAID-mClover-P2A-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-O-FUW-KDM2B-P2A-mCherry

    Plasmid

    #203852

    Purpose

    Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    KDM2B (KDM2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. YA1529: pAAV_hSyn-QuasAr3-P2A-CheRiff

    Plasmid

    #107700

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    QuasAr3-P2A-CheRiff
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tet-O-FUW-FOSL2-P2A-mCherry

    Plasmid

    #203860

    Purpose

    Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    FOSL2 (FOSL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-SlugCBE-NNG-P2A-puro

    Plasmid

    #214355

    Purpose

    Expresses SlugCBE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCBE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDEST mfap4:icre-p2a-tomato

    Plasmid

    #135217

    Purpose

    Cre coexpressed with tdTomato in macrophages

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Insert

    mfap4 promoter with iCre and Tomato
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTol2-nbt-Electra2-P2A-mScarlet

    Plasmid

    #196114

    Purpose

    zebrafish expression of nbt-Electra2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. XLone-Puro SPI1 P2A mCherry

    Plasmid

    #140029

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CAG-iRFP682-P2A-GFP

    Plasmid

    #197153

    Purpose

    Expresses the protein of iRFP682-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    iRFP682-P2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

    Plasmid

    #190153

    Purpose

    Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only AA #1-94

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

    Plasmid

    #170220

    Purpose

    Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    T14A, Y15F

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. XLone-BSD SPI1 P2A mCherry

    Plasmid

    #140031

    Purpose

    Tunable and temporal expression control of SPI1 and mCherry

    Depositor

    Xiaoping Bao

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV-tdTomato-P2A-paCaMKII(K42M)

    Plasmid

    #165433

    Purpose

    Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    tdTomato-P2A-paCaMKII(K42M)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XLone-BSD Cas13d P2A mCherry

    Plasmid

    #155183

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and mCherry

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tet-O-FUW-NR4A3-P2A-mCherry

    Plasmid

    #203863

    Purpose

    Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    NR4A3 (NR4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge