We narrowed to 75,869 results for: Rest

1. pX459-HypaCas9-mR2-CfANLN_sgRNA

   Plasmid

   #183879

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX459-HypaCas9-MYH10_sgRNA

   Plasmid

   #183887

   Purpose

   pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459-HypaCas9-mR2-H2BC11_sgRNA

   Plasmid

   #183881

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX459-HypaCas9-mR2-MYH10_sgRNA

   Plasmid

   #183886

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-MNTS-1-p75

   Plasmid

   #182945

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1-p75 (NTF3, NT3 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter and enhancer

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-MNTS-1

   Plasmid

   #182944

   Purpose

   To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR

   Depositor

   Pantelis Tsoulfas

   Article

   Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

   Insert

   MNTS-1 (NTF3 Synthetic, Human, Homo sapiens)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   This is the Human NT-3 with mutations on the matu…

   Promoter

   CMV promoter and enhancer

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-sgCTRL-1

   Plasmid

   #83934

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-sgCTRL-2

   Plasmid

   #83935

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-sgCTRL-3

    Plasmid

    #83936

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mTurquoise2-RnBarr1

    Plasmid

    #137795

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. shCTL-mlpx-neo

    Plasmid

    #65233

    Purpose

    encodes a non-targeting control shRNA

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    non-coding shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTYB-pLAC-F112S

    Plasmid

    #48727

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Phenylalanine 12 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pTYB1-pLAC-I98S

    Plasmid

    #48723

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 98 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pTYB1-pLAC-I73S

    Plasmid

    #48722

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 73 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 73 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pTYB1-pLAC-V69S

    Plasmid

    #48721

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 69 from Valine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Valine 69 to Serine

    Promoter

    T7

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PKT2/CLP-AKT

    Plasmid

    #20281

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    AKT (AKT1 Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PT3.5/CMV-EGFRvIII

    Plasmid

    #20280

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    EGFRvII (EGFR Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    See depositor comments below.

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-Flag-Sintbad

    Plasmid

    #234668

    Purpose

    expresses SINTBAD (TBKBP1) in mammalian cells

    Depositor

    Lienhard Schmitz

    Article

    Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDH_CMV_TFAM_mKate2

    Plasmid

    #167331

    Purpose

    Expresses mKate2 fused to full length TFAM in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWPI-N3ICD

    Plasmid

    #185524

    Purpose

    Constitutive overexpression of human NOTCH3 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH3 intracellular domain (NOTCH3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH_blast_MCS_Nard_GFP_LAMIN

    Plasmid

    #167340

    Purpose

    Expresses GFP fused to lamin A (LA) in mammalian cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLKO-RB1-shRNA63

    Plasmid

    #25641

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 63 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO-RB1-shRNA19

    Plasmid

    #25640

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 19 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pWPI-N1ICD

    Plasmid

    #185525

    Purpose

    Constitutive overexpression of human NOTCH1 intracellular domain

    Depositor

    Sean Palecek, Eric Shusta

    Article

    Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

    Insert

    NOTCH1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-HA-p53 2KR

    Plasmid

    #78799

    Purpose

    To overexpress p53 2KR in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K291R, K292R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pEMS1932

    Plasmid

    #79781

    Purpose

    High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple259
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple259 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pEMS1937

    Plasmid

    #79782

    Purpose

    High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple260
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple260 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Use

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSEMS-CV SUy-Halo7Tag

    Plasmid

    #108933

    Purpose

    Selflabeling tag at ATP Synthase

    Depositor

    Karin Busch

    Article

    Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

    Insert

    ATP synthase F1 subunit gamma (ATP5F1C Human)
    

    Use

    Tags

    Halo7 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 1099 pcDNA GFP FKHR AAA

    Plasmid

    #9023

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCIneoMyc UNC119B

    Plasmid

    #128333

    Purpose

    Mammalian expression vector of Myc-tagged human UNC119B

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

    Insert

    UNC119B (UNC119B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Use

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

40. alpha-0 CFP in pcDNA3.1

    Plasmid

    #36396

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein alpha 0 (Gnao1 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted into the KpnI-SpeI sites engineered …

    Promoter

    cmv

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only