Addgene

We narrowed to 158,755 results for: Gene

Showing: 1551 - 1600 of 158755 results

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JD135

Plasmid

#229491

Purpose

Ef1a promoter driving expression of adenoviral 100k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

100k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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JD181

Plasmid

#229495

Purpose

Ef1a promoter driving expression of adenoviral 23k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

23k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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JD73

Plasmid

#229482

Purpose

Adenoviral VA RNA I & II genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA I & II

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

wt promoter

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA63_HelperΔgIII

Plasmid

#190433

Purpose

M13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

M13 genes I-V and VII-XI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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JD108

Plasmid

#229494

Purpose

Adenoviral DeltaF6 plasmid variant devoid of fiber, U exon and pVIII sequences

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

multiple genes

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD182

Plasmid

#229488

Purpose

Ef1a promoter driving expression of adenoviral L4-22k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

L4-22k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD143

Plasmid

#229492

Purpose

CMV promoter driving expression of adenoviral E4orf6-6/7 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4orf6-6/7

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD81

Plasmid

#229481

Purpose

Adenoviral VA RNA II gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA II

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

wt promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD183

Plasmid

#229489

Purpose

Ef1a promoter driving expression of adenoviral L4-33k gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

L4-33k

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
JD92

Plasmid

#229478

Purpose

CMV promoter driving expression of adenoviral E4orf6 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4orf6

Use

AAV and Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCambia3301-AtU6-MAR1

Plasmid

#210757

Purpose

Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

Depositor

Daisuke Miki

Article

Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

Insert

AtU6-26 pro::MAR1 sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA325

Plasmid

#214742

Purpose

Medium copy glmS-nadE plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV013

Plasmid

#214739

Purpose

Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TST-EGFP-GPIDAF

Plasmid

#213712

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TST-EGFP-GPIBY55

Plasmid

#213711

Purpose

For overexpression of a gene, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an SV40 promoter.

Depositor

Qilai Huang

Article

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Use

Tags

Twin-strep-tag and V5-tag

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dErbB4

Plasmid

#197357

Purpose

A knock-out vector for dog ErbB4.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB4 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dErbB1

Plasmid

#197354

Purpose

A knock-out vector for dog ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-dErbB2

Plasmid

#197355

Purpose

A knock-out vector for dog ErbB2.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB2 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX458-dErbB3

Plasmid

#197356

Purpose

A knock-out vector for dog ErbB3.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

A gRNA targeting the dog ERBB3 gene and the cDNA of CRISPR-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
XLone-mARG2

Plasmid

#173792

Purpose

Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpN, F G L S K J U

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

TRE3G promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
XLone-GvpB

Plasmid

#173798

Purpose

Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpB

Use

Tags

mCherry (K187N -please see depositor comments)

Expression

Mammalian

Mutation

Promoter

TRE3G promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUMP24_T24_pRham-ilvA-relE

Plasmid

#201531

Purpose

Expressing synthetic overlapping gene, ilvA-relE. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3412

Plasmid

#193109

Purpose

Minimal promoter of NtPCPS2 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1027 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minNtPCPS2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3410

Plasmid

#193107

Purpose

Minimal promoter of NtTA29 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1477 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minNtTA29

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3411

Plasmid

#193108

Purpose

Minimal promoter of At2S3 gene containing 84bp upstream the transcription start site and the 5'UTR region (from GB0029 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minAt2S3

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3409

Plasmid

#193106

Purpose

Minimal promoter of slANS gene (Solyc10g076660) containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlANS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3408

Plasmid

#193105

Purpose

Minimal promoter of slCHS1 gene (Solyc09g091510) containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlCHS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3414

Plasmid

#193111

Purpose

Minimal promoter of AnGPDA gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB007 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minAnGPDA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB2566

Plasmid

#193104

Purpose

Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlDFR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GB3413

Plasmid

#193110

Purpose

Minimal promoter of PcPAF gene containing 62bp upstream the transcription start site and the 5'UTR region (from FB029 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minPcPAF

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV40_mD6Ertd527e-HA

Plasmid

#192222

Purpose

Expresses HA-tagged murine D6Ertd527e gene in mammalian cells

Depositor

Petr Svoboda

Article

Petrzilek et al BMC Biol. 2022 Dec 8;20(1):272. doi: 10.

Insert

mD6Ertd527e (D6Ertd527e Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 391

Plasmid

#138059

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23101- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 392

Plasmid

#138053

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23102- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH41

Plasmid

#138035

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-7GT-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG TAG AAA

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 406

Plasmid

#138067

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23100- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 405

Plasmid

#138066

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23119- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 398

Plasmid

#138065

Purpose

A novel variant of the Anderson promoter using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23101-3TG- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 397

Plasmid

#138064

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23115- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 396

Plasmid

#138063

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23110- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 395

Plasmid

#138062

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23109- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 394

Plasmid

#138061

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23108- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 393

Plasmid

#138060

Purpose

Encodes Anderson promoter variant using mRFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

J23105- pSEVA-331Bb- RFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MYMH 75

Plasmid

#138052

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-WTRBS-GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY31CBiT

Plasmid

#127761

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

Convergent LacI-TetR Toggle

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYD1A

Plasmid

#127759

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in divergent orientation

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYRC-D1A

Plasmid

#127753

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

RFP, CFP, Divergent Orientation

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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