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178,218 results

Showing: 6381 - 6400 of 178218 results

  4. pDG462

    Plasmid
    #100903
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100903-plasmid-map-sequence-id-196607

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  8. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91854-plasmid-map-sequence-id-176529

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  9. pcDNA3-hPR-A

    Plasmid
    #89119
    Purpose
    mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933
    Depositor
    Elizabeth Wilson
    Publication
    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human progesterone receptor-A (PGR Human)
    Expression
    Mammalian
    Mutation
    human progesterone receptor-A, equivalent to huma…
    Promoter
    CMV
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89119-plasmid-map-sequence-id-175729

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  15. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

    Plasmid
    #85583
    Purpose
    cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)
    Depositor
    Paul Sternberg
    Publication
    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    Prab-3-GAL4-SK(DBD)-VP64
    Expression
    Worm
    Promoter
    Prab-3-GAL4-SK(DBD)-VP64
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85583-plasmid-map-sequence-id-170931

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  17. pScarlessHD-sfGFP-DsRed

    Plasmid
    #80811
    Purpose
    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    sfGFP-piggyBac-DsRed
    Use
    Unspecified
    Tags
    sfGFP
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80811-plasmid-map-sequence-id-152800

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  18. Talin-CS

    Plasmid
    #83377
    Purpose
    Control FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Publication
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted at C-terminal o…
    Promoter
    CMV
    Available since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83377-plasmid-map-sequence-id-158762

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  20. p5E-elavl3

    Plasmid
    #75025
    Purpose
    Can be used to drive pan neuronal expression in zebrafish.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    elavl3
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75025-plasmid-map-sequence-id-141916

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Showing: 6381 - 6400 of 178218 results