19,658 results

1. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159816

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pinducer 20 DN-KASH

   Plasmid

   #125554

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   tetracycline responsive element

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH21-BNA-Luc

   Plasmid

   #187924

   Purpose

   Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
   

   Expression

   Mammalian

   Promoter

   RNA polymease I Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-SNAP-KanMX6

   Plasmid

   #87024

   Purpose

   Tag S. pombe genes in C terminal with SNAP

   Depositor

   Julien Berro

   Article

   Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

   Insert

   SNAP
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Expression

   Plant

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFlagCMV2-UbcH8

   Plasmid

   #12442

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   UbcH8 (UBE2L6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pJC005.em

   Plasmid

   #182738

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSP3545

   Plasmid

   #46888

   Purpose

   Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible Expression

   Depositor

   Gary Dunny

   Article

   Bryan et al Plasmid. 2000 Sep;44(2):183-90.

   Type

   Empty backbone

   Use

   Nisin-inducible expression; gram-positive bacteri…

   Expression

   Bacterial

   Promoter

   PnisA

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CrmA

   Plasmid

   #11832

   Depositor

   Guy Salvesen

   Article

   Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

   Insert

   CrmA (CrmA Cowpox virus)
   

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. BE4-Gam

    Plasmid

    #100806

    Purpose

    Expresses BE4-Gam in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

    Insert

    BE4-Gam
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 SIRT7_H187Y

    Plasmid

    #53151

    Purpose

    expression vector for catalytically inactive human Sirtuin7

    Depositor

    Katrin Chua

    Article

    Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

    Insert

    Sirt7 (SIRT7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H187Y, catalytic inactive

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-ATF5-PROX1-HNF1A

    Plasmid

    #149725

    Purpose

    expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

    Depositor

    Baisong Lu

    Article

    Lu et al Biomaterials. 2021 Feb;269:120668. doi:

    Insert

    ATF5-PROX1-HNF1A (ATF5 Human)
    

    Use

    Lentiviral

    Tags

    P2A, T2A

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST-CMV-N-EGFP

    Plasmid

    #122842

    Purpose

    N-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PU6::unc-119_sgRNA

    Plasmid

    #46169

    Purpose

    unc-119 targeting sgRNA

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    unc-119 targeting sgRNA (unc-119 Synthetic)
    

    Use

    CRISPR

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available Since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 WT B-Myb

    Plasmid

    #25965

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (MYBL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-Cdk9

    Plasmid

    #28102

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Tags

    3x HA

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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