We narrowed to 8,762 results for: sgrna

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pgRNAkan-glpR

Plasmid

#169873

Purpose

Single guide RNA plasmid for Cas9 targeting glpR in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards glpR in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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KA2601_eSpCas9-G2P

Plasmid

#124203

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLG_GI4

Plasmid

#111595

Purpose

GI Library Vector

Depositor

Jonathan Weissman

Article

Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR

Plasmid

#100008

Purpose

Expresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genome

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lsg-LRRK2-1

Plasmid

#199277

Purpose

nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 nick

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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LentiU6-hNEUROD1 gRNA_1-MS2-Puro

Plasmid

#192676

Purpose

Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human NEUROD1 activating gRNA #1 (NEUROD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_TOP1

Plasmid

#68423

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pX459-sg-tdTomato90/816

Plasmid

#179918

Purpose

Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

Depositor

Indeever Madireddy, Johan Sosa

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

U6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Expression

Bacterial and Yeast

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Nsp2-SmuCas9_AAV

Plasmid

#192144

Purpose

Expresses Nsp2-SmuCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

Nsp2-SmuCas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174119

Purpose

Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Promoter

mU6 and UbC

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

Plasmid

#192681

Purpose

Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_A2S-8

Plasmid

#188654

Purpose

AAV vector expressing SaAID-2S and gRNA

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S--nSaCas9-UGI
Sa-sgRNA

Use

AAV and CRISPR

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

Scp1 and U6

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#2

Plasmid

#183123

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-2

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pQCi

Plasmid

#154345

Purpose

Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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