We narrowed to 10,413 results for: plasmids 101

1. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

   Plasmid

   #113549

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSyn-Aion-citrine

   Plasmid

   #192569

   Purpose

   AAV-vector for optogenetic long-term silencing of neurons

   Depositor

   Simon Wiegert

   Article

   Rodriguez-Rozada et al Commun Biol. 2022 Jul 9;5(1):687. doi: 1

   Insert

   Aion
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

   Promoter

   human synapsin

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

   Plasmid

   #99144

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFlag-HaloTag-EZH2 HDR - pDY053

   Plasmid

   #171108

   Purpose

   Homology directed repair plasmid for generating HaloTag-EZH2 cells

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV:: HA-hM4Dnrxn

   Plasmid

   #52525

   Purpose

   Expresses N-terminal HA tagged hM4Dnrxn

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Insert

   HA-hM4Dnrxn (CHRM4 Human, Synthetic)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-AcGFP-P2A-hBRD4S

    Plasmid

    #197882

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-Stim1-D76A

    Plasmid

    #186622

    Purpose

    Encodes Stim1 mutant D76A fused to YFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    D76A

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CAG-RORb2

    Plasmid

    #73999

    Purpose

    Overexpression of RORb2

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    RORb2 ORF (Rorb Mouse)
    

    Tags

    none

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    Tet-On

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmKO-AF8

    Plasmid

    #118224

    Purpose

    Kusabira- Orange-CENP-A expression plasmid

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Mutat Res. 2008 Nov 17;657(1):56-62. doi

    Insert

    CENP-A (CENPA Human)
    

    Tags

    Kusabira orange

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. KLF17-CRa1-Lsg-MS2

    Plasmid

    #247476

    Purpose

    sgRNA1 for KLF17 activation cloned into LsgRNA-MS2 backbone

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    sgRNA1 targeting KLF17 promoter (KLF17 Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. KLF17-CRa2-Lsg-MS2

    Plasmid

    #247477

    Purpose

    gRNA2 for KLF17 activation cloned into LsgRNA-MS2 backbone

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    sgRNA2 targeting KLF17 promoter (KLF17 Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pN3-sTRAIL

    Plasmid

    #154246

    Purpose

    Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

    Depositor

    Jordan Green

    Article

    Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

    Insert

    Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand
    

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

    Plasmid

    #208429

    Purpose

    Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(hMTOR_2b)-PGKpuro2ABFP-W

    Plasmid

    #208430

    Purpose

    Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    MTOR (MTOR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4_10xUAS-CMVmin-BC0410-luc2

    Plasmid

    #227121

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0410

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0410 and a luciferase reporter gene
    

    Expression

    Mammalian

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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