We narrowed to 67,877 results for: TOR

1. pcDNA3.1-Flag-gp130dimer-stm2585

   Plasmid

   #174395

   Purpose

   Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   IL6ST cytosolic domain and sarA chimera
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Flag-stm2585

   Plasmid

   #174391

   Purpose

   S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

   Depositor

   Dennis Ko

   Article

   Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

   Insert

   stm2585
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p1.2-Zeo-hVKORC1

   Plasmid

   #162776

   Purpose

   Human vitamin K oxidoreductase expression

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

   Insert

   VKORC1 vitamin K epoxide reductase complex subunit 1 (VKORC1 Human)
   

   Expression

   Mammalian

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB6-Cdk6-T2A-TagRFPT-dre-PBD*-FNF-TK

   Plasmid

   #141452

   Purpose

   B6J Cdk6-T2A-TagRFPT-dre-PBD* allele targeting vector, low copy number

   Depositor

   Mario Capecchi

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Mouse Targeting; Dre / rox

   Tags

   C-terminal fusion of T2A-TagRFPT-dre-PBD*

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrichoGate-17

   Plasmid

   #160717

   Purpose

   TrpC Terminator bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   tryptophan synthase transcription terminator (TtrpC) bordered by BsaI restriction sites
   

   Use

   Synthetic Biology

   Mutation

   Incorporation of BsaI restriction sites

   Available Since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR-v1-sgNADK_1

   Plasmid

   #163462

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting NADK

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 1 targeting NADK (NADK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK422

   Plasmid

   #155373

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK423

   Plasmid

   #155374

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159961

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159965

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159964

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159958

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159957

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159960

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159959

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-CMV-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159963

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-Casp-2L

    Plasmid

    #157932

    Purpose

    Over expression of Casp-2L

    Depositor

    Baoji Xu

    Article

    Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

    Insert

    Casp2 (Casp2 Mouse)
    

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mTurquoise2-RnBarr2

    Plasmid

    #137798

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSTAP-seq_fly-4xUAS

    Plasmid

    #125149

    Purpose

    Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Stap-seq screening vector

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pKD250.2

    Plasmid

    #125077

    Purpose

    Expression of torR under PLtetO-1, output promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    torR (torR )
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET41a iGluSnFR E25R

    Plasmid

    #119831

    Purpose

    Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25R
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25R

    Promoter

    T7

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

    Plasmid

    #104462

    Purpose

    Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

    Insert

    mCherry-subT391A-FHA1-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    CAG

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGS_C_3xFLAG

    Plasmid

    #101653

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal 3xFLAG-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, 3xFLAG tag
    

    Tags

    3xFLAG tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. BB1_12_pGPM1

    Plasmid

    #98499

    Purpose

    BB1 with insert: promoter PGPM1

    Depositor

    Brigitte Gasser, Michael Sauer, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GPM1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GST-AR-1-36

    Plasmid

    #104192

    Purpose

    Expresses human AR aa 1-36 in pGEX-5x-1 bact expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-36 (AR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-HA-REDD1 S137D (#628)

    Plasmid

    #99944

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S137D

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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