2,226 results

1. pLVX-CMV-Galpha-i3 G183S ONE-GO

   Plasmid

   #204338

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   Galpha-i3 G183S ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G183S mutation in Galpha-i3 (renders it RGS insen…

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCMV-dCpf1-eBE

   Plasmid

   #107688

   Purpose

   Expresses dLbCpf1-eBE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-C2gamma

   Plasmid

   #21215

   Depositor

   Tobias Meyer

   Article

   Codazzi et al Curr Biol. 2001 Jul 24. 11(14):1089-97.

   Insert

   GFP-pcDNA3-PKCgamma-c2 (Prkcg Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The C2-domain from PKCgamma (aa170-260) plus flan…

   Promoter

   Available since

   July 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-BE-VQRCas9

   Plasmid

   #101740

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, VQR mutations

   Promoter

   CMV

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA type III TGF beta receptor (R3-OFF)

   Plasmid

   #10913

   Depositor

   Bob Weinberg

   Article

   Wang et al Cell. 1991 Nov 15. 67(4):797-805.

   Insert

   R3-OFF (Tgfbr3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pBa.Kif5c 1-559.GFP

   Plasmid

   #45059

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

   Insert

   Kif5c (Kif5c Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   motor domain aa1-559

   Promoter

   Chicken Beta Actin

   Available since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.1 HA pCAGGS

   Plasmid

   #206076

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

   Depositor

   Annette Dolphin

   Article

   Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/B-actin

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-GCaMP3bright

   Plasmid

   #74423

   Purpose

   Express fluorescent calcium indicator GCaMP3bright in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3bright
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D392A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

   Plasmid

   #123615

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A/K34A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A/K34A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3XFLAG-CMV 7.1_syn6

    Plasmid

    #50012

    Purpose

    Encodes syntaxin 6 with a N-terminal 3X FLAG tag

    Depositor

    David Hackstadt

    Article

    Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

    Insert

    syntaxin 6 (Stx6 Rat)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV ORANGE Gria1-HaloTag KI

    Plasmid

    #139655

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-APOBEC1-YTHmut

    Plasmid

    #200159

    Purpose

    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTHmut
    

    Use

    Tags

    6xHis, HA, and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-DIO-OMOR-eYFP

    Plasmid

    #66847

    Purpose

    Expresses Opto-MOR in a Cre dependent manner

    Depositor

    Michael Bruchas

    Article

    Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

    Insert

    Opto Mu opioid receptor
    

    Use

    AAV and Cre/Lox

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG.PKCzeta.T410A

    Plasmid

    #10801

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Activation loop inactive: T410A (Thr 410 to Ala)

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSV Sport SREBP-1c dom neg

    Plasmid

    #8885

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

    Insert

    SREBP-1c (Srebf1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y305A amino acid substitution in the active versi…

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-rSyntenin1

    Plasmid

    #89431

    Purpose

    mammalian expressio of EGFP tagged rat Syntenin1

    Depositor

    Fritz Rathjen

    Article

    Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

    Insert

    SDCBP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. YFP-DBD

    Plasmid

    #14874

    Depositor

    Alexandra Newton

    Article

    Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

    Insert

    DAG binding domain (Prkcb Rat)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Y123W

    Promoter

    Available since

    Aug. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLV302

    Plasmid

    #119943

    Purpose

    Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. KIF5C(1-560)-2xmCherry-EF(C)

    Plasmid

    #61664

    Purpose

    Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI-syn-fRGECO2

    Plasmid

    #125244

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W44Y, D432A

    Promoter

    synapsin

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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