Addgene

We narrowed to 8,796 results for: CAG

Showing: 961 - 990 of 8796 results

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PB CAG-F-3xSV40pA-F-td-sfGFP

Plasmid

#133570

Purpose

CAG tdGFP FLP/FRT reporter piggyBac transposon (3x SV40pA stop)

Depositor

Mario Capecchi

Insert

Tandem dimer superfolder GFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184V

Plasmid

#106187

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Tags

Expression

Mutation

GltI: A184V

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-emiRFP703

Plasmid

#197205

Purpose

Expresses the protein of emiRFP703 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP703

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Use

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Elavl2IR

Plasmid

#120518

Purpose

dsRNA expression

Depositor

Petr Svoboda

Insert

Elavl2 (Elavl2 Mouse)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mCherry

Plasmid

#87664

Purpose

retroviral vector encoding cre dependent expression of membrane mCherry

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

Mem-mCherry

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GCaMP2-actin

Plasmid

#18928

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

GCaMP2-Actin (ACTB Chicken)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
DN1-Gli3 in AINGpCAGGS

Plasmid

#121970

Purpose

medium Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-864)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Omicron.BA.5-RBD

Plasmid

#213478

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/Omicron.BA.5 RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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prCAG-RBS"m1"-sfGFP

Plasmid

#191400

Purpose

Expression of 47 rCAG repeats fused to a translational unit over-expressing superfolder GFP using a strong RBS. The RBS contains mutation (m1)

Depositor

Ariel Lindner

Article

Guo et al Cell. 2022 Sep 29;185(20):3823-3837.e23.

Insert

47xCAG RBS"m1"-sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCAGGS/EG5-RBD

Plasmid

#213410

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/EG5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-3xHAtag-SP-PTPRO

Plasmid

#191696

Purpose

For expression coding sequencing of PTPRO

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, 3xHAtag and full length coding sequence of PTPRO

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mGSDMD1-251

Plasmid

#111565

Purpose

Express ELANE-cleaved mouse GSDMD in mammalian cells

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (Gsdmd Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only
pCAG-SP-CLSTN2-3xHAtag

Plasmid

#191693

Purpose

For expression coding sequencing of CLSTN2

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

signal peptide, full length coding sequence of CLSTN2 and 3xHAtag

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-eArch3.0-mRuby2-ST

Plasmid

#109135

Purpose

Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eArch3.0-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-S-B.1.1.529-FLAG

Plasmid

#180774

Purpose

B.1.1.529 variant S-protein with c-terminal FLAG tag

Depositor

Daniel Conway

Insert

S-protein B.1.1.529

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. aureus)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Mutation

Promoter

U6 promoter;

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-mRuby2

Plasmid

#164093

Purpose

expression of mRuby2-tagged proteins under CAG promoter

Depositor

Maik Hintze

Insert

mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miRNT

Plasmid

#177808

Purpose

A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP-int

Plasmid

#19822

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
Cav2.2 D122A HA pCAGGS

Plasmid

#206097

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV/B-actin

Available since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS2

Plasmid

#128106

Purpose

Express HA tagged Nrxn1α-LNS2

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS2 (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.S72A

Plasmid

#106203

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Tags

Expression

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

CAG

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CRY2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG GST DEPDC5

Plasmid

#164983

Purpose

DEPDC5 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

DEPDC5 (DEPDC5 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS::AtHAP2-YFP

Plasmid

#132960

Purpose

Express AtHAP2 full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

At HAP2::YFP

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGEN-SBP-DICER1

Plasmid

#50558

Purpose

purification of Dicer1 from mammalian cells

Depositor

Yukihide Tomari

Article

Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

Insert

Dicer1 (Dicer1 Mouse)

Use

Tags

SBP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only