2,226 results

1. SNAP-23

   Plasmid

   #186686

   Purpose

   Expresses SNAP-23 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   SNAP23
   

   Use

   Tags

   GST and thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV8-hSyn-flex-miR30-eGFP-shCrh

   Plasmid

   #132715

   Purpose

   Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   GFP-shCrh (Crh Rat)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin 1

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMS-eGFP-RTP801

   Plasmid

   #65057

   Purpose

   Expression of RTP801 in mammalian cells

   Depositor

   Cristina Malagelada, Lloyd Greene

   Article

   Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

   Insert

   DDIT4 (Ddit4 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

4. CMV-mCyRFP2-CREB

   Plasmid

   #137003

   Purpose

   mCyRFP2-CREB donor for R-CREB

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Use

   Tags

   mCyRFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEAK10-His-Myc-Kal12

   Plasmid

   #25442

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal12
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pORANGE Doc2A-GFP KI

   Plasmid

   #131478

   Purpose

   Endogenous tagging of Doc2a: C-terminal (amino acid position: L402)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Doc2a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAd-PV-NLS-CD-DsRed

   Plasmid

   #16345

   Depositor

   Anton Bennett

   Article

   Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

   Insert

   PV-DsRed-NLS-CD (Pvalb Rat)
   

   Use

   Adenoviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Functional Ca2+binding site CD was inactivated by…

   Promoter

   Available since

   Feb. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn-eGFP-SynaptoZip

   Plasmid

   #122525

   Purpose

   AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

   Depositor

   Antonio Malgaroli

   Article

   Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

   Insert

   eGFP-SynaptoZip (Vamp2 Rat)
   

   Use

   AAV

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cx43K258stop-msfGFP

   Plasmid

   #69023

   Purpose

   Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   V206K monomerized super folder GFP

   Expression

   Mammalian

   Mutation

   delete codons 258-381 and fuse in frame to monome…

   Promoter

   CMV

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMK282 (mCherry2-Bsr)

    Plasmid

    #72798

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FLAG.PKCzeta.T410E

    Plasmid

    #10804

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Activation loop active: T410E (Thr 410 to Glu)

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pCMV5-myc-Erk2

    Plasmid

    #39222

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2001 Jul 13;276(28):26509-1

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pRK7-HA-S6K1-F5A-E389-deltaCT

    Plasmid

    #8990

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A, T389E, stop codon at amino acid 402

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pBK-EE-BE3

    Plasmid

    #85175

    Purpose

    Expresses EE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-ERK2 K52R

    Plasmid

    #118298

    Purpose

    Expression of Rat ERK2

    Depositor

    Philip Stork

    Article

    Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

    Insert

    ERK2 K52R (Mapk1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K52R

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBluescript-Evf1

    Plasmid

    #99479

    Purpose

    Subcloning of the Evf1 long non-coding RNA

    Depositor

    Jhumku Kohtz

    Article

    Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

    Insert

    Evf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Emerald-Lysosomes-20

    Plasmid

    #56476

    Purpose

    Localization: Lysosome Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    Emerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pFSynW SV2A HaloTag

    Plasmid

    #218545

    Purpose

    Lentiviral expression of SV2A HaloTag

    Depositor

    Edwin Chapman

    Insert

    SV2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCI SV2A pHluorin

    Plasmid

    #218543

    Purpose

    Mamallian Expression of SV2A pHluorin

    Depositor

    Edwin Chapman

    Insert

    SV2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S42F, L199P

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-evoA1-seBE4max-IRES

    Plasmid

    #174699

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    evoA1-seBE4max-IRES
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    UGI-E11K

    Promoter

    CMV

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV5M/rDlg1

    Plasmid

    #28259

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Dlg1 I3
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pCMV-dCpf1-BE-YE

    Plasmid

    #107686

    Purpose

    Expresses dLbCpf1-BE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. tdTurboRFP-Lysosomes-20

    Plasmid

    #58061

    Purpose

    Localization: Lysosome Membrane, Excitation: 553, Emission: 576

    Depositor

    Michael Davidson

    Insert

    LAMP1
    

    Use

    Tags

    tdTurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pcDNA3 βarr1 S412D Flag

    Plasmid

    #42196

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    β-arrestin 1 S412D (Arrb1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S412D

    Promoter

    CMV

    Available since

    Jan. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pAAV-syn-SnFR-gamma8-minWPRE

    Plasmid

    #165498

    Purpose

    AAV for glutamate reporter fused to TARP gamma-8

    Depositor

    Andrew Plested

    Article

    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

    Insert

    iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218865

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-eBE-S3

    Plasmid

    #101093

    Purpose

    Expresses eBE-S3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

    Insert

    eBE-S3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOC2 Tubb3-GFP KI

    Plasmid

    #183432

    Purpose

    CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge