Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,325 results for: membrane gfp

Clear keywords
Showing: 1281 - 1320 of 1325 results

  2. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available Since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

    Enlarge

  3. ORAI1-YFP T184M

    Plasmid
    #114181
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114181-plasmid-map-sequence-id-218676

    Enlarge

  4. ORAI1-YFP V107M

    Plasmid
    #114180
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114180-plasmid-map-sequence-id-218674

    Enlarge

  19. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234873-plasmid-map-sequence-id-469050

    Enlarge

  20. ORAI1-YFP V107M-L273D-L276D

    Plasmid
    #114183
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M/L273D/L276D
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114183-plasmid-map-sequence-id-218685

    Enlarge

  21. ORAI1-YFP T184M-L273D-L276D

    Plasmid
    #114184
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M/L273D/L276D
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114184-plasmid-map-sequence-id-220961

    Enlarge

  33. NK85

    Plasmid
    #176611
    Purpose
    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.
    Depositor
    Meghan Morrissey
    Article
    Kern et al Elife. 2021 Jun 3;10. pii: 68311. doi: 1
    Insert
    FCERG
    Use
    Lentiviral
    Tags
    EGFP and HA, SNAP-tag
    Expression
    Mammalian
    Mutation
    C25A
    Available Since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176611-plasmid-map-sequence-id-362592

    Enlarge

  36. MLKS2delTMexp

    Plasmid
    #130863
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of transmembrane domain
    Available Since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130863-plasmid-map-sequence-id-255916

    Enlarge

  37. pCYC1pr-GNSI (LEU)

    Plasmid
    #111450
    Purpose
    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    111450-plasmid-map-sequence-id-211605

    Enlarge

  39. pSUC2::GNSI (NrsR)

    Plasmid
    #121537
    Purpose
    This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
    Depositor
    Alexey Merz
    Article
    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1
    Inserts
    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    NrsR
    Tags
    mGFP5
    Expression
    Yeast
    Mutation
    M109I (Naturally occurring SNP) and The first 21 …
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121537-plasmid-map-sequence-id-235299

    Enlarge

Showing: 1281 - 1320 of 1325 results