Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 66,158 results for: %s

Clear keywords
Showing: 641 - 660 of 66158 results

  6. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Publication
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52319-plasmid-map-sequence-id-237583

    Enlarge

  7. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid
    #46168
    Purpose
    Codon optimized Cas9_SV40 NLS with intron
    Depositor
    John Calarco
    Publication
    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    codon optimized Cas9_SV40 NLS with intron
    Use
    CRISPR
    Mutation
    intron inserted at position 3113-3163, and SV40 N…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    July 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46168-plasmid-map-sequence-id-65793

    Enlarge

  16. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid
    #73498
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73498-plasmid-map-sequence-id-364403

    Enlarge

Showing: 641 - 660 of 66158 results