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pCasSA

Plasmid

#98211

Purpose

CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

Depositor

Quanjiang Ji

Publication

Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

Plasmid

#46168

Purpose

Codon optimized Cas9_SV40 NLS with intron

Depositor

John Calarco

Publication

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

codon optimized Cas9_SV40 NLS with intron

Use

CRISPR

Mutation

intron inserted at position 3113-3163, and SV40 N…

Promoter

eef-1A.1 (eft-3)

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

47 Articles

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Publication

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Publication

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Publication

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pDEST-periHisMBP

Plasmid

#11086

Depositor

David Waugh

Publication

Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

Type

Empty backbone

Tags

6xHis, MBP, and MBP signal peptide

Expression

Bacterial

Promoter

Tac (lactose/IPTG inducible)

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLifeAct_mScarlet_N1

Plasmid

#85054

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pET-MBP-mSA2

Plasmid

#52319

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Publication

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, MBP, and TEV

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSF3-ultraID

Plasmid

#172878

Purpose

expresses ultraID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Publication

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pBABE-puro HGF

Plasmid

#10901

Depositor

Bob Weinberg

Publication

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

HGF (HGF Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMVTNT PINK1 C-myc

Plasmid

#13314

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lambda Phosphatase

Plasmid

#79748

Purpose

Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

Lambda Phosphatase

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pME-Cas9

Plasmid

#63154

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

Depositor

Leonard Zon

Publication

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9

Use

CRISPR; Tol2 middle entry vector for gateway

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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sfGFP-N1

Plasmid

#54737

Purpose

Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

Depositor

Michael Davidson, Geoffrey Waldo

Publication

Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

Type

Empty backbone

Tags

sfGFP

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

21 Articles

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pAAV-MCS2

Plasmid

#46954

Purpose

Cloning plasmid for the generation of Knock-In in human cells using rAAV.

Depositor

Steve Jackson

Publication

Britton et al J Cell Biol. 2013 Jul 29.

Type

Empty backbone

Use

AAV

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Publication

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGP-CMV-jGCaMP8m

Plasmid

#162372

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8m

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV hUbC-dCas9-T2A-GFP

Plasmid

#53191

Purpose

Co-expresses human optimized S. pyogenes dCas9 and GFP

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMSCV-FlagMLL-pl-ENL(5613)

Plasmid

#20873

Depositor

Robert Slany

Publication

Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

Insert

MLL (KMT2A, MLLT1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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