We narrowed to 15,599 results for: LEA

1. pJRoC30_Th3.10

   Plasmid

   #188067

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM-U6-reRNA-mmPCSK9

   Plasmid

   #205634

   Purpose

   reRNA guide targeting Mus Musculus PCSK9

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-mmPCSK9 (Pcsk9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPSt_35Spr-GFP-t35S

   Plasmid

   #203592

   Purpose

   Fully assembled Plant STARR-seq plasmid with the 35S terminator.

   Depositor

   Stan Fields

   Article

   Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

   Inserts

   CaMV 35S promoter + Zm00001d041672 5'UTR
   eGFP
   CaMV 35S terminator
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-ArgiNLS-miRFP670

   Plasmid

   #220600

   Purpose

   Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   miRFP670
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-ArgiNLS-mKate2

   Plasmid

   #220599

   Purpose

   Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   mKate2
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-ArgiNLS-AausFP1

   Plasmid

   #220595

   Purpose

   Constitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   AausFP1
   

   Use

   AAV

   Tags

   ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

   Plasmid

   #131683

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Inserts

   EGFP-KASH
   SpCas9 sgRNA vs mouse GRID1
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   EF1a and mU6

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

   Plasmid

   #195018

   Purpose

   An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

   Depositor

   Christopher Richie

   Article

   Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

   Inserts

   CCGTATAGGCGGCTGCCCAA
   EGFP-KASH
   TTCCGTGGTGGGCAACGTAG
   

   Use

   AAV

   Tags

   KASH domain of Nesprin2

   Expression

   Mutation

   Promoter

   EF1a, hU6, and mU6

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSAVED-CHAT

    Plasmid

    #214041

    Purpose

    Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. KU70-eGFP-SUN1ΔN

    Plasmid

    #213516

    Purpose

    Expresses human KU70-eGFP-SUN1ΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    KU70-eGFP-SUNΔN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-cFOS-FRB-VP16-P2A-EGFP

    Plasmid

    #210510

    Purpose

    expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    FRB-VP16-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cFos

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. DRD2-V2tail-TevN-BLITz-TetR-FKBP

    Plasmid

    #210514

    Purpose

    expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    DRD2-V2tail-TevN-BLITz-TetR-FKBP
    

    Use

    Tags

    HA signal FALG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. WT_IFL_G1A-T2A-A28C_R

    Plasmid

    #200185

    Purpose

    WT PA14 I-F Leader and G1A-T2A-A28C Repeat

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

    Insert

    Wildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G1A-T2A-A28C in the I-F repeat

    Promoter

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBactin-AcGFP-CI

    Plasmid

    #196849

    Purpose

    Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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