178,737 results
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Plasmid#54090PurposeLocalization: Focal Adhesions/Membrane, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEzrin (EZR Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby-Lifeact-7
Plasmid#54560PurposeLocalization: Actin, Excitation: 558, Emission: 605DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-mRuby
ExpressionMammalianAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
delta DE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50468PurposeGs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInsertrM3D-IRES-mCitrine
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CaMKIIa-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50466PurposeGq-coupled hM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-ms-Klf2
Plasmid#50786Purposeretroviral expression of mouse Klf2DepositorAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
flag-hKLF6 (1006)
Plasmid#49488Purposeexpresses human flag tagged KLF6 in mammalian cellsDepositorAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF5/FRT/DEST-3xHA/mGli3/Flag
Plasmid#51246PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-CDepositorAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-ReaChR-citrine
Plasmid#50956PurposeReaChR-citrine in lentival transfer vector under human synapsin promoterDepositorInsertReaChR-citrine
UseLentiviralTagscitrinePromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN565
Plasmid#49990Purposeexpresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible controlDepositorInsertT7 RNAP*
UseSynthetic BiologyTagsumuD degradation tagExpressionBacterialMutationR632SPromoterpTac-symmetricAvailable sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG-HA-CAD
Plasmid#46238PurposeFor expression of HA and FLAG-tagged murine CAD in mammalian cellsDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
TagsFLAG and HAExpressionMammalianPromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
TagsHAExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO-sh-hSC
Plasmid#46896Purposeexpression of scrambled shRNADepositorInsertscrambled
UseLentiviral and RNAiExpressionMammalianAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorPublicationInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
BECLIN-HA WT
Plasmid#46993PurposeExpresses Beclin1 in mammalian cellsDepositorAvailable sinceAug. 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSV-PKI-v2
Plasmid#45066PurposeExpression of PKI (PKA inhibitor)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMP-dependent protein kinase inhibitor alpha
ExpressionMammalianPromoterRSVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ909
Plasmid#44480DepositorInsertMinimal Mos1, unc-119, MCS
UseMultiple cloning site vectorAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA Depdc5 pRK5
Plasmid#46327DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDepdc5 (DEPDC5 Human)
TagsHAExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX Src-SH2
Plasmid#46510DepositorInsertSrc (SRC Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 142-252PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-HDAC4 3SA
Plasmid#45637DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHDAC4 (HDAC4 Human)
TagsEGFPExpressionMammalianMutation3SAPromoterCMVAvailable sinceJune 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF13E
Plasmid#42942DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEN- Human L1 element (L1RE1 Human)
TagsEGFP (opposite strand)ExpressionMammalianMutationAgeI-BclI fragment swapped with nt 1927-3708 from…PromoterCMV MIE, L1 5'UTRAvailable sinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF12J
Plasmid#42943DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEN+ Human L1 element (L1RE1 Human)
TagsEGFP (opposite strand)ExpressionMammalianMutationAgeI-BclI fragment swapped with nt 1927-3708 from…PromoterCMV MIE, L1 5'UTRAvailable sinceApril 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-HBXIP
Plasmid#42326DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBXIP (LAMTOR5 Human)
TagsFlagExpressionMammalianPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVLAD6
Plasmid#41850DepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2 Notch1 ICv-6MT
Plasmid#41730DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNotch-1 Intracellular Domain (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 protein beginning at Vali…Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHMGWA-Pa14Csy4H29A
Plasmid#41092DepositorInsertCsy4
TagsHis6-MBPMutationHis29Ala mmutation to abolish ribonuclease activi…PromoterT7Available sinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABEpuro-ERBB2 A775_G776insYVMA
Plasmid#40982DepositorAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMIEG3-JunB
Plasmid#40349DepositorAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AMPK-γ1-HA
Plasmid#40605DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK-γ1 (Prkag1 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by