19,658 results

1. pCI flag PCAF

   Plasmid

   #8941

   Depositor

   Yoshihiro Nakatani

   Article

   Yang et al Nature. 1996 Jul 25. 382(6589):319-24.

   Insert

   PCAF (KAT2B Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEXfrt-SaCas9-U6-sgRNA

   Plasmid

   #124845

   Purpose

   Vector for FLP-dependent expression of SaCas9 with gRNA

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   SauCas9 gRNA scaffold
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Egr1

   Plasmid

   #11729

   Depositor

   Eileen Adamson

   Article

   Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

   Insert

   Egr-1 (Egr1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154786

   Purpose

   Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Fly, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and mCherry

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. V406 4HA-CKIe(K38R)

   Plasmid

   #13725

   Depositor

   David Virshup

   Article

   Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

   Insert

   casein kinase 1 epsilon (CSNK1E Human)
   

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   kinase-inactive due to K38R mutation

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLmMBP-10

   Plasmid

   #72348

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-SUMO-hGSDMD

   Plasmid

   #111559

   Purpose

   Express human GSDMD in E.coli

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (GSDMD Human)
   

   Tags

   His, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nuclear Myosin I YFP

   Plasmid

   #60241

   Purpose

   Expresses nuclear isoform of myosin IC attached to YFP and an NLS

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP rbFOX1

    Plasmid

    #63085

    Purpose

    Mammalian expression of EGFP tagged FOX1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    RBFOX1 (RBFOX1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV TRE3G Neo GFP-Progerin

    Plasmid

    #118710

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV TRE3G (TET-ON)

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMU-NUCr

    Plasmid

    #61168

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet3-alphaTubulin_C1

    Plasmid

    #189768

    Purpose

    Construct for labelling microtubules in mammalian cells with mScarlet3-alphatubulin

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-alphaTubulin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pminiTol2

    Plasmid

    #31829

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR33.4r

    Plasmid

    #63198

    Purpose

    Expresses Cph8, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    Cph8
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. plRL19

    Plasmid

    #163634

    Purpose

    L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Insert

    L5 Integrase
    

    Expression

    Bacterial

    Promoter

    Mycobacterial Optimized Promoter (MOP) and Tet-On

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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