165,528 results

1. pAXY0013

   Plasmid

   #205582

   Purpose

   NpuDnaE(C)_HYG(53-341)_RUBY

   Depositor

   Xiaohan Yang

   Article

   Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

   Insert

   NpuDnaE(C)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-c-Met

   Plasmid

   #31786

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Use

   Entry vector

   Available Since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG-HHARI

   Plasmid

   #17450

   Depositor

   Dong-Er Zhang

   Article

   Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

   Insert

   HHARI (ARIH1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 5, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCAR_Cas9-hygro_GFP

   Plasmid

   #162075

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-hygromycin B phosphotransferase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB167

   Plasmid

   #101672

   Purpose

   Opto-T7RNAP*(563), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563), equal expr.
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-HA-Nedd4/2795

   Plasmid

   #11426

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D661N compared to GenBank reference sequence U966…

   Promoter

   CMV

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA flag PPAR gamma

   Plasmid

   #8895

   Purpose

   Mammalian expression of flag-tagged PPAR gamma

   Depositor

   Bruce Spiegelman

   Article

   Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

   Insert

   PPAR gamma (Pparg Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Neo-Bam APC

   Plasmid

   #16507

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-Sos1

    Plasmid

    #32920

    Depositor

    Dafna Bar-Sagi

    Article

    Yang et al J Biol Chem. 1995 Aug 4;270(31):18212-5.

    Insert

    SOS1 (SOS1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3_dCas7-11

    Plasmid

    #197605

    Purpose

    Expresses inactive Cas7-11 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    dCas7-11
    

    Expression

    Mammalian

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYX122-mtGFP

    Plasmid

    #45048

    Depositor

    Benedikt Westermann

    Article

    Westermann et al Yeast. 2000 Nov;16(15):1421-7.

    Insert

    mtGFP
    

    Expression

    Yeast

    Promoter

    TPI

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCMV_53BP1_FLAG

    Plasmid

    #237345

    Purpose

    transient overexpression in mammalian cells

    Depositor

    Michele Pagano

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM5188

    Plasmid

    #120087

    Purpose

    Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

    Depositor

    Susan Golden

    Article

    Chen et al Microbiology. 2016 Dec;162(12):2029-2041

    Insert

    pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
    

    Use

    Other

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.10 Dub3

    Plasmid

    #72684

    Purpose

    luciferase reporter with Dub3 promoter

    Depositor

    Domenico Maiorano

    Article

    van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

    Insert

    Dub3 promoter (Usp17le Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Dub3

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPICZ-pre-pro-alphaf(I)-E2-Crimson

    Plasmid

    #117660

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf(I)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti CMV/TO Neo DEST (685-3)

    Plasmid

    #17292

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pINDUCER21-RORA

    Plasmid

    #51303

    Purpose

    Expression of RORA after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    RAR-related orphan receptor A (RORA Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Mutation

    full length ORF

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Available Since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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25. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUC57KAN-40XALFA

    Plasmid

    #233118

    Purpose

    40X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUC57KAN-20XALFA

    Plasmid

    #233112

    Purpose

    20X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Mutation

    None

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRSET-QUE2m

    Plasmid

    #129350

    Purpose

    ATP measurement

    Depositor

    Hiroyuki Noji

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-2m
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. AICSDP-43: GJA1-mEGFP

    Plasmid

    #109120

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

    Depositor

    Allen Institute for Cell Science

    Insert

    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. pSFFV_mNG3K(1-10)

    Plasmid

    #157993

    Purpose

    Lentiviral vector of constitutive expression of mNeonGreen3K(1-10)

    Depositor

    Bo Huang

    Article

    Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

    Insert

    pSFFV_mNG3K(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    G27D, D42E, M128I, K153M, T170I

    Promoter

    SFFV

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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