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pLVX-HA-UBE3B(C1036A)-IRES-Puro

Plasmid

#107348

Purpose

Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(C1036A) (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

changed Cysteine 1036 to Alanine

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pST_DSUP_RAMVA (pBS0353)

Plasmid

#185189

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mTcte1-PA

Plasmid

#176469

Purpose

Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

t-complex-associated testis expressed 1 (Tcte1 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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KG#205

Plasmid

#110881

Purpose

Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

Inserts

rab-3 promoter
pde-4d (isoform d) D448N cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

changed Asp 448 to Asn

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.VTN.C-HA

Plasmid

#154849

Purpose

Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Vitronectin (Vtn Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pgRGFP

Plasmid

#82695

Purpose

Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

Depositor

Alan Mullen

Article

Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

PGK, U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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HMGA2-FLAG

Plasmid

#25409

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Retroviral; Chip

Tags

FLAG

Expression

Mammalian

Mutation

None

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (kinase dead)

Plasmid

#29544

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed lysine 751 to methionine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
RET-SHC

Plasmid

#139617

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET (RET Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRV FOXP3 WWRR

Plasmid

#13625

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

WWRR: T359W, N361W, E399R, E401R

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pMIR-CDH1 wtUTR

Plasmid

#25038

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

Plasmid

#101298

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-T402A

Plasmid

#81179

Purpose

Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

T402A

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_FANCM

Plasmid

#221379

Purpose

Expresses FANCM in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long UTR

Plasmid

#21649

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 long UTR (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLV Reep3-V5

Plasmid

#175119

Purpose

Lentiviral expression of mouse Reep3-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep3 (Reep3 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_AG

Plasmid

#148763

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK1

Plasmid

#197108

Purpose

Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (TAOK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Tmem9-V5

Plasmid

#175151

Purpose

Lentiviral expression of mouse Tmem9-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem9 (Tmem9 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-YOD1

Plasmid

#85663

Purpose

Expression of human YOD1 with N-terminal mCherry tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip

Plasmid

#178054

Purpose

myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCas4_1_2_VA

Plasmid

#186438

Purpose

Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

Plasmid

#129538

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAH18

Plasmid

#154397

Purpose

Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-WT

Plasmid

#114063

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-WT (TTK Human)

Tags

His6

Expression

Insect

Mutation

none

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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