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pET31b-T7-Spinach2

Plasmid

#79783

Purpose

Expresses Spinach2 under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 HA C9ORF72 op

Plasmid

#74156

Purpose

Express HA tagged optimized C9ORF72 in mammalian cells

Depositor

Nicolas Charlet-Berguerand

Insert

C9ORF72 (C9orf72 Synthetic, Human)

Tags

HA

Expression

Mammalian

Mutation

optimized sequence

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Ct

Plasmid

#79158

Purpose

Expresses the Ct biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo

Plasmid

#209636

Purpose

Expression miniTurbo in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Dp

Plasmid

#79159

Purpose

Expresses the Dp biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Dp

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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p2-39

Plasmid

#118086

Purpose

ZB-linker-split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

ZB-linker-split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p5-71

Plasmid

#118085

Purpose

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

N-29-1 split N-terminal T7 RNAP variant-linker-ZA expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCT2-HRP

Plasmid

#44021

Depositor

Dane Wittrup

Article

Lipovsek et al Chem Biol. 2007 Oct;14(10):1176-85.

Insert

HRP

Tags

HA and c-myc

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only
pJRK246

Plasmid

#173741

Purpose

donor plasmid for SapTrap assembly

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

wrt-2 promoter

Use

Other

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

EGFP and HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor1

Plasmid

#49352

Purpose

Promoterless luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

None

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor3

Plasmid

#49354

Purpose

pSV40mod-luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

SV40mod

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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PRS_8-Jaws-tdTomato

Plasmid

#192593

Purpose

AAV-vector for optogenetic silencing of noradrenergic neurons

Depositor

Simon Wiegert

Article

Maheu et al bioRxiv 2025.03.26.644382

Insert

Jaws

Use

AAV

Tags

tdTomato-ER2

Expression

Mammalian

Promoter

PRSX8

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBADZ-HisCre

Plasmid

#111187

Purpose

Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

Depositor

Tim Richmond

Article

Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

Insert

Cre recombinase

Tags

His tag

Expression

Bacterial

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP49

Plasmid

#22586

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP49 (USP49 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pAC-Luc

Plasmid

#33154

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pKAR2-Br512g3.1

Plasmid

#171200

Purpose

Expresses Br512g3.1 polymerase in E.coli

Depositor

Andrew Ellington

Article

Bhadra et al medRxiv 2022.08.09.22278605

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (vHP47…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBL069B

Plasmid

#194802

Purpose

cCadC-GCN4* under the control of Ptet

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-GCN4*

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-hRluc

Plasmid

#24348

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

hRluc

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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Copia Renilla Control

Plasmid

#38093

Depositor

Philip Beachy

Article

Lum et al Science. 2003 Mar 28;299(5615):2039-45.

Insert

renilla luciferase

Use

Luciferase

Expression

Insect

Promoter

copia

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pBL090J

Plasmid

#195045

Purpose

cCadC-CCB3.5 under the control of PBAD

Depositor

Tina Wang

Article

Lee et al ACS Synth Biol. 2022 Oct 21;11(10):3198-

Insert

cCadC-CCB3.5

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP42

Plasmid

#22603

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP42 (USP42 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-dGreenPy1

Plasmid

#239063

Purpose

Mammalian expression of green fluorescent pyruvate control biosensor dGreenPy1

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

dGreenPy1

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3

Plasmid

#136010

Purpose

psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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R26P-M2rtTA

Plasmid

#47381

Purpose

Rosa 26 targeting vector

Depositor

Rudolf Jaenisch

Article

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Inserts

M2rtTA
pgk-puro

Use

Mouse Targeting

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
GST-PRMT1

Plasmid

#34699

Depositor

Steven Clarke

Article

Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

Insert

PRMT1 (Prmt1 Rat)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP45

Plasmid

#22605

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP45 (USP45 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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